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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Continuous Flow Synthesis of Chiral Amines in Organic Solvents: Immobilization of E. coli Cells Containing Both omega-Transaminase and PLP

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Autor(es):
Andrade, Leandro H. [1] ; Kroutil, Wolfgang [2] ; Jamison, Timothy F. [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] MIT, Dept Chem, Cambridge, MA 02139 - USA
[2] Graz Univ, Dept Chem Organ & Bioorgan Chem, A-8010 Graz - Austria
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ORGANIC LETTERS; v. 16, n. 23, p. 6092-6095, DEC 5 2014.
Citações Web of Science: 66
Resumo

E. coli cells containing overexpressed (R)-selective omega-transaminase and the cofactor PLP were immobilized on methacrylate beads suitable for continuous flow applications. The use of an organic solvent suppresses leaching of PLP from the cells; no additional cofactor was required after setting up the packed-bed reactor containing the biocatalyst (omega-TA-PLP). Non-natural ketone substrates were transformed in flow with excellent enantioselectivity (>99% ee). Features of this novel system include high-throughput (30-60 min residence time), clean production (no quench, workup, or purification required), high enzyme stability (the packed-bed reactor can be continuously operated for 1-10 days), and excellent mass recovery. (AU)

Processo FAPESP: 13/04540-0 - Transformação de dióxido de carbono usando métodos enzimáticos sobre condições de fluxo contínuo
Beneficiário:Leandro Helgueira de Andrade
Linha de fomento: Bolsas no Exterior - Pesquisa