Hyaluronidase Modulates Inflammatory Response and Accelerates the Cutaneous Wound Healing

Autor(es)

Fronza, Marcio ^{[1, 2]} ; Caetano, Guilherme F. ^[3] ; Leite, Marcel N. ^[3] ; Bitencourt, Claudia S. ^[1] ; Paula-Silva, Francisco W. G. ^[1] ; Andrade, Thiago A. M. ^[3] ; Frade, Marco A. C. ^[3] ; Merfort, Irmgard ^[4] ; Faccioli, Lucia H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14049 Ribeirao Preto, SP - Brazil

^[2] Univ Vila Velha, Dept Farm, Vila Velha, ES - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, Div Dermatol, BR-14049 Ribeirao Preto, SP - Brazil

^[4] Univ Freiburg, Dept Pharmaceut Biol & Biotechnol, D-79106 Freiburg - Germany

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 9, n. 11 NOV 13 2014.
• Citações Web of Science: 23

Resumo

Hyaluronidases are enzymes that degrade hyaluronan an important constituent of the extracellular matrix. They have been used as a spreading agent, improving the absorption of drugs and facilitating the subcutaneous infusion of fluids. Here, we investigated the influence of bovine testes hyaluronidase (HYAL) during cutaneous wound healing in in vitro and in vivo assays. We demonstrated in the wound scratch assay that HYAL increased the migration and proliferation of fibroblasts in vitro at low concentration, e.g. 0.1 U HYAL enhanced the cell number by 20%. HYAL presented faster and higher reepithelialization in in vivo full-thickness excisional wounds generated on adult Wistar rats back skin already in the early phase at 2nd day post operatory compared to vehicle-control group. Wound closured area observed in the 16 U and 32 U HYAL treated rats reached 38% and 46% compared to 19% in the controls, respectively. Histological and biochemical analyses supported the clinical observations and showed that HYAL treated wounds exhibited increased granulation tissue, diminished edema formation and regulated the inflammatory response by modulating the release of pro and anti-inflammatory cytokines, growth factor and eicosanoids mediators. Moreover, HYAL increased gene expression of peroxisome proliferator-activated receptors (PPAR) gamma and PPAR beta/delta, the collagen content in the early stages of healing processes as well as angiogenesis. Altogether these data revealed that HYAL accelerates wound healing processes and might be beneficial for treating wound disorders. (AU)

• Processo FAPESP: 09/07169-5 - Mediadores lipídicos como reguladores da resposta imune
• Beneficiário: Lúcia Helena Faccioli
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/23992-3 - Avaliação da atividade biológica da enzima hialuronidase e sua possível participação no processo de cicatrização cutânea
• Beneficiário: Marcio Fronza
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado