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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

MALDI-MS of flavonoids: a systematic investigation of ionization and in-source dissociation mechanisms

Texto completo
Autor(es):
Silva, Denise B. [1] ; Lopes, Norberto P. [1]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, Nucleo Pesquisas Prod Nat & Sintet, BR-14040903 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Journal of Mass Spectrometry; v. 50, n. 1, p. 182-190, JAN 2015.
Citações Web of Science: 8
Resumo

Matrix assisted laser desorption ionization (MALDI) is a technique widely employed in the analysis of proteins and peptides, and nowadays it has also been applied to small molecules. There is little significant information regarding the in-source dissociation processes on MALDI for natural products. Twenty-six flavonoids (flavanones, flavones and flavonols) were analyzed by MALDI using different methods (with different matrices) and without matrix to comprehend the in-source reactions and establish good analysis methods for these compounds. Depending on the class, structure and the laser intensity applied, methoxylated flavonoid aglycones can eliminate methyl radicals (CH3) in the source, such as flavonols, but lithium 2,4-dihydroxybenzoate matrix suppresses the CH3 eliminations and retro-Diels-Alder cleavages in the source. All of the flavonoid O-glycosides evaluated herein eliminated the sugar in source, even in the presence of the matrix, and its product radical ions ({[}M-H-sugar](-)) were observed in the negative mode. The flavone C-glycosides suffered intense dissociation, which was reduced by the addition of a matrix and the application of low laser intensity, mainly in the negative mode. Depending on the hydroxyl substituents, the {[}M-H-H](-) ion was observed with variable relative intensity in the spectra. Copyright (c) 2015 John Wiley \& Sons, Ltd. (AU)

Processo FAPESP: 12/18031-7 - Aplicação de MALDI-MS/MS na análise da vicenina-2 e correlatos: da compreensão dos processos ionizantes à geração de imagens
Beneficiário:Denise Brentan da Silva
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 09/54098-6 - EMU: aquisição de um espectrômetro de massas para geração de imagens na Central de Espectrometria de Massas (mass-facility) da FCFRP-USP: estudos de localização molecular de substâncias biologicamente ativas
Beneficiário:Norberto Peporine Lopes
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários