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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening

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Autor(es):
Guazzaroni, Maria-Eugenia [1] ; Silva-Rocha, Rafael [2] ; Ward, Richard John [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, FFCLRP, Dept Quim, BR-14049 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, FMRP, Dept Bioquim & Imunol, BR-14049 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo de Revisão
Fonte: MICROBIAL BIOTECHNOLOGY; v. 8, n. 1, p. 52-64, JAN 2015.
Citações Web of Science: 22
Resumo

There is a growing demand for enzymes with improved catalytic performance or tolerance to process-specific parameters, and biotechnology plays a crucial role in the development of biocatalysts for use in industry, agriculture, medicine and energy generation. Metagenomics takes advantage of the wealth of genetic and biochemical diversity present in the genomes of microorganisms found in environmental samples, and provides a set of new technologies directed towards screening for new catalytic activities from environmental samples with potential biotechnology applications. However, biased and low level of expression of heterologous proteins in Escherichia coli together with the use of non-optimal cloning vectors for the construction of metagenomic libraries generally results in an extremely low success rate for enzyme identification. The bottleneck arising from inefficient screening of enzymatic activities has been addressed from several perspectives; however, the limitations related to biased expression in heterologous hosts cannot be overcome by using a single approach, but rather requires the synergetic implementation of multiple methodologies. Here, we review some of the principal constraints regarding the discovery of new enzymes in metagenomic libraries and discuss how these might be resolved by using synthetic biology methods. (AU)

Processo FAPESP: 13/04125-2 - Novas ferramentas genéticas para Archaea: validação funcional e modelagem da rede regulatória de RNA não codificantes de Halobacterium salinarum
Beneficiário:Rafael Silva Rocha
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/18850-2 - Identificação, caracterização e engenharia de enzimas que degradam a parede celular das plantas
Beneficiário:Richard John Ward
Linha de fomento: Auxílio à Pesquisa - Temático