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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cecropia pachystachya extract attenuated the renal lesion in 5/6 nephrectomized rats by reducing inflammation and renal arginase activity

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Autor(es):
Maquiaveli, Claudia C. [1] ; da Silva, Edson R. [2] ; Rosa, Lara C. [2] ; Francescato, Heloisa D. C. [1] ; Lucon Junior, Joao Francisco [2] ; Silva, Cleonice G. A. [1] ; Casarini, Dulce E. [3] ; Ronchi, Fernanda A. [3] ; Coimbra, Terezila M. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Physiol, Sch Med Ribeirao Preto, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Vet Med, Sch Anim Sci & Food Engn, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Med, Div Nephrol, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Ethnopharmacology; v. 158, n. A, p. 49-57, DEC 2 2014.
Citações Web of Science: 4
Resumo

Ethnopharmacological relevance: The plant Cecropia pachystachya Trecul has been used in Brazilian folk medicine to treat hypertension, bladder and kidney inflammation and renal diseases. The aim of this study was to evaluate the potential of the aqueous fraction from the ethanolic extract of Cecropia pachystachya (FCP) in the management of hypertension, inflammation and progressive renal disease in rats submitted to 5/6 nephrectomy. Materials and methods: Thirty male Wistar rats submitted to 5/6 nephrectomy (5/6 NE) were untreated (NE) or treated (NE+FCP) with the FCP (0.5 g/kg/day). The treatment started 15 days after surgery, and the rats were followed for a period of 60 days. Systolic blood pressure (SBP) and albuminuria were evaluated from 15-60 days after the surgical procedure. Function and estructural renal changes, TGF-beta (transforming growth factor beta), MCP-1 (monocyte chemoattractant protein-1) and nitric oxide (NO) urinary excretion were analyzed. Expression and activity of the renal enzymes arginase (ARG), angiotensin converting enzyme (ACE), and MAP kinase p-JNK expression also were analyzed. Results: The nephrectomized rats developed progressive albuminuria and increased SBP that was less intense in the treated group. There was a reduction in the glomerular filtration rate (GFR) in the nephrectomized rats, which was attenuated by treatment with FCP extract. The treatment with FCP also attenuated the histological changes, reduced the expression and activity of renal arginase, the number of macrophages (ED-1 positive cells) and the p-JNK expression in the renal cortex of the rats submitted to 5/6 NE. The urinary excretion of TGF-beta was less intense in the treated group and was associated with the reduction of the expression and activity of the renal arginase. Conclusions: These results suggest that the reduction of renal arginase activity, p-JNK and TGF-beta expression can explain the mechanism by which the treatment with C. pachystachya reduced the inflammation and improved renal function. This study presents the potential use of Cecropia pachystachya in the treatment of chronic renal diseases. (C) 2014 Elsevier Ireland Ltd. All rights reserved. (AU)

Processo FAPESP: 11/50391-0 - Efeito do extrado bruto e frações de Cecropia pahystachya (embaúba) na evolução da lesão renal provocada pela nefrectomia a 5/6
Beneficiário:Terezila Machado Coimbra
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/17059-5 - Caracterização de inibidores naturais e estudo da relação estrutura-atividade de flavonóides isolados sobre a enzima arginase de Leishmania amazonensis
Beneficiário:Edson Roberto da Silva
Linha de fomento: Auxílio à Pesquisa - Regular