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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Novel and cross-amplified microsatellite loci for the critically endangered Sao Paulo marsh antwren Formicivora paludicola (Aves: Thamnophilidae)

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Autor(es):
de Camargo, Crisley [1] ; Costa, Mariellen C. [2] ; Del Rio, Glaucia C. [3] ; Gibbs, H. Lisle [4] ; Glenn, Travis C. [5] ; Bagal, Ujwal [5] ; Silveira, Luis F. [6] ; Wasko, Adriane P. [1] ; Francisco, Mercival R. [2]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Inst Biociencias, Dept Genet, BR-18618970 Botucatu, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Ciencias Ambientais, BR-18052780 Sorocaba, SP - Brazil
[3] Univ Sao Paulo, Inst Biociencias, Dept Zool, BR-04218970 Sao Paulo, SP - Brazil
[4] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 - USA
[5] Univ Georgia, Dept Environm Hlth Sci, Athens, GA 30602 - USA
[6] Univ Sao Paulo, Museu Zool, Secao Aves, BR-04218970 Sao Paulo, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: CONSERVATION GENETICS RESOURCES; v. 7, n. 1, p. 129-131, MAR 2015.
Citações Web of Science: 3
Resumo

The So Paulo marsh antwren (Formicivora paludicola) is a critically endangered bird endemic to marshes in the metropolitan region of So Paulo city, Brazil. The total population is estimated to be around 300 individuals, distributed among 15 small (< 50 ha) fragments, suggesting that loss of genetic variability may affect the long-term viability of this species. To develop genetic tools for gaining information on effective population sizes, inbreeding and gene flow between populations, we describe nine polymorphic microsatellite loci isolated from a F. paludicola library using next-generation sequencing. We report on levels of variation in these novel microsatellites and eight additional heterologous loci in these birds. Expected (H (E)) and observed (H (O)) heterozygosities averaged 0.72 and 0.70, respectively, and the number of alleles per locus ranged from 3 to 10. These loci will permit evaluation of whether artificial translocations are necessary for long-term viability of this rare bird. (AU)

Processo FAPESP: 12/09105-7 - Análise populacional do bicudinho-do-brejo-paulista, Formicivora aff. acutirostris (Passeriformes: Thamnophilidae): uma espécie recém descoberta no Alto Tietê, São Paulo, Brasil
Beneficiário:Crisley de Camargo
Linha de fomento: Bolsas no Brasil - Mestrado