Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Didanosine-loaded chitosan microspheres optimized by surface-response methodology: A modified ``Maximum Likelihood Classification'' approach formulation for reverse transcriptase inhibitors

Texto completo
Autor(es):
da Silva, Classius Ferreira [1] ; Severino, Patricia [2, 3, 4] ; Martins, Fernanda [4] ; Santana, Maria Helena A. [4] ; Souto, Eliana B. [5, 6, 7]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema - Brazil
[2] Univ Tiradentes, BR-49010390 Aracaju - Brazil
[3] Inst Technol & Res, BR-49010390 Aracaju - Brazil
[4] Univ Estadual Campinas, Dept Biotechnol Proc, Sch Chem Engn, BR-13083970 Campinas, SP - Brazil
[5] Univ Coimbra FFUC, Dept Pharmaceut Technol, Fac Pharm, Polo Ciencias Saude, P-3000548 Coimbra - Portugal
[6] Univ Coimbra, Polo Ciencias Saude, Ctr Neurosci & Cell Biol, P-3000548 Coimbra - Portugal
[7] Univ Coimbra, Polo Ciencias Saude, Inst Biomed Imaging & Life Sci CNC IBILI, P-3000548 Coimbra - Portugal
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: BIOMEDICINE & PHARMACOTHERAPY; v. 70, p. 46-52, MAR 2015.
Citações Web of Science: 2
Resumo

Didanosine-loaded chitosan microspheres were developed applying a surface-response methodology and using a modified Maximum Likelihood Classification. The operational conditions were optimized with the aim of maintaining the active form of didanosine (ddI), which is sensitive to acid pH, and to develop a modified and mucoadhesive formulation. The loading of the drug within the chitosan microspheres was carried out by ionotropic gelation technique with sodium tripolyphosphate (TPP) as cross-linking agent and magnesium hydroxide (Mg(OH)(2)) to assure the stability of ddI. The optimization conditions were set using a surface-response methodology and applying the ``Maximum Likelihood Classification'', where the initial chitosan concentration, TPP and ddI concentration were set as the independent variables. The maximum ddI-loaded in microspheres (i.e. 1433 mg of ddI/g chitosan), was obtained with 2% (w/v) chitosan and 10% TPP. The microspheres depicted an average diameter of 11.42 mu m and ddI was gradually released during 2 h in simulated enteric fluid. (C) 2015 Elsevier Masson SAS. All rights reserved. (AU)

Processo FAPESP: 08/00421-8 - Incorporação e avaliação imunológica de antigenos tumorais incorporados em nanopartículas lipídicas sólidas e nanocarreadores lipídicos sólidos
Beneficiário:Patricia Severino
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/20801-2 - Encapsulação de polimixina B em NLS para incorporação em hidrogéis mucoadesivos para tratamento de mucosite oral
Beneficiário:Patricia Severino
Linha de fomento: Bolsas no Brasil - Pós-Doutorado