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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

DNA Methylation Levels of Melanoma Risk Genes Are Associated with Clinical Characteristics of Melanoma Patients

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Autor(es):
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de Araujo, Erica S. S. [1] ; Pramio, Dimitrius T. [1] ; Kashiwabara, Andre Y. [2] ; Pennacchi, Paula C. [3] ; Maria-Engler, Silvya S. [3] ; Achatz, Maria I. [1, 4] ; Campos, Antonio H. J. F. M. [5] ; Duprat, Joao P. [6] ; Rosenberg, Carla [7] ; Carraro, Dirce M. [1] ; Krepischi, Ana C. V. [1, 7]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] AC Camargo Canc Ctr, Int Res Ctr, BR-01508010 Sao Paulo, SP - Brazil
[2] Fed Technol Univ Parana, BR-86300000 Cornelio Procopio, PR - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508000 Sao Paulo, SP - Brazil
[4] AC Camargo Canc Ctr, Dept Oncogenet, BR-01509010 Sao Paulo, SP - Brazil
[5] AC Camargo Canc Ctr, Dept Pathol, BR-01509010 Sao Paulo, SP - Brazil
[6] AC Camargo Canc Ctr, Skin Canc Dept, BR-01509010 Sao Paulo, SP - Brazil
[7] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolutionary Biol, BR-05508090 Sao Paulo, SP - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: BIOMED RESEARCH INTERNATIONAL; 2015.
Citações Web of Science: 4
Resumo

In melanoma development, oncogenic process is mediated by genetic and epigenetic mutations, and few studies have so far explored the role of DNA methylation either as predisposition factor or biomarker. We tested patient samples for germline CDKN2A methylation status and found no evidence of inactivation by promoter hypermethylation. We have also investigated the association of clinical characteristics of samples with the DNA methylation pattern of twelve genes relevant for melanomagenesis. Five genes (BAP1, MGMT, MITF, PALB2, and POT1) presented statistical association between blood DNA methylation levels and either CDKN2A-mutation status, number of lesions, or Breslow thickness. In tumors, five genes (KIT, MGMT, MITF, TERT, and TNF) exhibited methylation levels significantly different between tumor groups including acral compared to nonacral melanomas and matched primary lesions and metastases. Our data pinpoint that the methylation level of eight melanoma-associated genes could potentially represent markers for this disease both in peripheral blood and in tumor samples. (AU)

Processo FAPESP: 13/07480-8 - Fatores genéticos e epigenéticos na etiologia do melanoma cutâneo
Beneficiário:Ana Cristina Victorino Krepischi
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/10785-5 - Investigação do perfil mutacional em melanomas cutâneos e impacto no padrão global de metilação de DNA
Beneficiário:Dimitrius Tansini Pramio
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 12/13963-9 - Perfil global de metilação do genoma na síndrome do melanoma familial
Beneficiário:Érica Sara Souza de Araújo
Linha de fomento: Bolsas no Brasil - Doutorado