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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Analysis of Genomic Regions of Trichoderma harzianum IOC-3844 Related to Biomass Degradation

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Autor(es):
Crucello, Aline [1] ; Sforca, Danilo Augusto [1] ; Crivelente Horta, Maria Augusta [1] ; dos Santos, Clelton Aparecido [1] ; Carvalho Viana, Americo Jose [1] ; Beloti, Lilian Luzia [1] ; Szymanski de Toledo, Marcelo Augusto [1] ; Vincentz, Michel [1, 2] ; Kuroshu, Reginaldo Massanobu [3] ; de Souza, Anete Pereira [1, 2]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 10, n. 4 APR 2 2015.
Citações Web of Science: 6
Resumo

Trichoderma harzianum IOC-3844 secretes high levels of cellulolytic-active enzymes and is therefore a promising strain for use in biotechnological applications in second-generation bioethanol production. However, the T. harzianum biomass degradation mechanism has not been well explored at the genetic level. The present work investigates six genomic regions (similar to 150 kbp each) in this fungus that are enriched with genes related to biomass conversion. A BAC library consisting of 5,760 clones was constructed, with an average insert length of 90 kbp. The assembled BAC sequences revealed 232 predicted genes, 31.5% of which were related to catabolic pathways, including those involved in biomass degradation. An expression profile analysis based on RNA-Seq data demonstrated that putative regulatory elements, such as membrane transport proteins and transcription factors, are located in the same genomic regions as genes related to carbohydrate metabolism and exhibit similar expression profiles. Thus, we demonstrate a rapid and efficient tool that focuses on specific genomic regions by combining a BAC library with transcriptomic data. This is the first BAC-based structural genomic study of the cellulolytic fungus T. harzianum, and its findings provide new perspectives regarding the use of this species in biomass degradation processes. (AU)

Processo FAPESP: 09/13185-3 - Análise da estrutura genômica de regiões do genoma do fungo Trichoderma harzianum contendo genes envolvidos na produção de enzimas com atividade celulolítica
Beneficiário:Aline Crucello
Linha de fomento: Bolsas no Brasil - Mestrado