Incidence of `Candidatus Liberibacter asiaticus'-Infected Plants Among Citrandarins as Rootstock and Scion Under Field Conditions

Autor(es)

Boava, Leonardo Pires ^[1] ; Duarte Sagawa, Cintia Helena ^[1] ; Cristofani-Yaly, Mariangela ^[1] ; Machado, Marcos Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Ctr Citricultura Sylvio Moreira, BR-13490970 Cordeiropolis Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHYTOPATHOLOGY; v. 105, n. 4, p. 518-524, APR 2015.
• Citações Web of Science: 7

Resumo

Huanglongbing (HLB), caused by the bacterium `Candidatus Liberibacter' spp., is currently one of the most serious diseases of citrus plants and has caused substantial economic losses. Thus far, there is no source of genetic resistance to HLB in the genus Citrus or its relatives. However, several studies have reported Poncirus trifoliata and some of its hybrids to be more tolerant to the disease. The main objective of this study was to report differences in the incidence of `Ca. L. asiaticus' infection in citrandarin plants, hybrids from Sunki mandarin (Citrus sunki (Hayata) hort. ex Tanaka), and trifoliate orange Rubidoux (P. trifoliata (L.) Raf.)), after conducting an extensive survey under field conditions. These hybrid plants were established for approximately 7 years in an area with a high incidence of `Ca. L. asiaticus'-infected plants. We selected two experimental areas (area A and area B), located approximately 10 m apart. Area A consists of Pera sweet orange (C. sinensis (L.) Osb.) grafted onto 56 different citrandarin rootstocks. Area B consists of citrandarin scions grafted onto Rangpur lime (C. limonia Osb.) rootstock. Bacteria in the leaves and roots were detected using real-time quantitative polymerase chain reaction. The incidence of `Ca. L. asiaticus'-infected plants was 92% in area A and 14% in area B. Because infected plants occurred in both areas, we examined whether the P. trifoliata hybrid rootstock influenced HLB development and also determined the distribution of `Ca. L. asiaticus' in Citrus tree tissues. Although this survey does not present evidence regarding the resistance of P. trifoliata and its hybrids in relation to bacteria or psyllids, future investigation, mainly using the most promising hybrids for response to `Ca. L. asiaticus', will help us to understand the probable mechanism of defense or identifying compounds in P. trifoliata and its hybrids that are very important as strategy to combat HLB. Details of these results are presented and discussed in this article. (AU)

• Processo FAPESP: 11/18605-0 - Obtenção e avaliação de novas variedades copas e porta-enxertos para citricultura de mesa
• Beneficiário: Mariângela Cristofani-Yaly
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/01917-2 - Genômica funcional e mapeamento genético de novos híbridos de citros tolerantes ao huanglongbing (HLB ou greening)
• Beneficiário: Leonardo Pires Boava
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/08435-5 - Resistência às doenças em citros: mapeamento genético e genômica funcional de novos híbridos
• Beneficiário: Mariângela Cristofani-Yaly
• Linha de fomento: Auxílio à Pesquisa - Regular