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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification

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Autor(es):
de Cassia Pereira, J. [1] ; Paganini Marques, N. [2] ; Rodrigues, A. [3] ; Brito de Oliveira, T. [3] ; Boscolo, M. [4] ; da Silva, R. [1] ; Gomes, E. [1] ; Bocchini Martins, D. A. [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP IBILCE, Lab Biochem & Appl Microbiol, Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP IQ, Lab Microbial Enzymes, Sao Paulo - Brazil
[3] Sao Paulo State Univ IB UNESP, Lab Ecol & Systemat Fungi, Sao Paulo - Brazil
[4] Sao Paulo State Univ IB UNESP, Lab Sucrochem & Analyt Chem, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Applied Microbiology; v. 118, n. 4, p. 928-939, APR 2015.
Citações Web of Science: 31
Assunto(s):Sacarificação   Glicerol   Fungos termófilos
Resumo

AimsTo obtain new cellulases and xylanases from thermophilic fungi; evaluate their potential for sugarcane bagasse saccharification. Methods and ResultsThirty-two heat-tolerant fungi were isolated from the environment, identified (morphological/molecular tools) and the production of the enzymes was evaluated by solid state fermentation using lignocellulosic materials as substrates. Myceliophthora thermophila JCP 1-4 was the best producer of endoglucanase (35751Ug(-1)), -glucosidase (4542Ug(-1)), xylanase (93111Ug(-1)) and avicelase (358Ug(-1)). These enzymes were most active at 55-70 degrees C and stable at 30-60 degrees C. Using crude enzymatic extract from M.thermophila JCP 1-4 to saccharify sugarcane bagasse pretreated with microwaves and glycerol, glucose and xylose yields obtained were 156 and 3513% (22 and 195g l(-1)), respectively. ConclusionsAll isolated fungi have potential to produce the enzymes; M.thermophila JCP 1-4 enzymatic extract have potential to be better explored in saccharification experiments. Pretreatment improved enzymatic saccharification, as sugar yields were much higher than those obtained from in natura bagasse. Significance and Impact of the StudyMyceliophthora thermophila JCP 1-4 produces avicelase (not commonly found among fungi; important to hydrolyse crystalline cellulose) and a -glucosidase resistant to glucose inhibition, interesting characteristics for saccharification experiments. (AU)

Processo FAPESP: 10/12624-0 - Aplicação de métodos físico-químicos e enzimáticos na sacarificação do bagaço de cana: estudos de microrganismos, processos fermentativos relacionados e métodos de hidrólise
Beneficiário:Eleni Gomes
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/02080-4 - Produção de etanol de bagaço de cana-de-açúcar pré-tratado com ozônio: estudo de inibidores, fermentação e leveduras industriais
Beneficiário:Roberto da Silva
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular