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MpSaci is a widespread gypsy-Ty3 retrotransposon highly represented by non-autonomous copies in the Moniliophthora perniciosa genome

Texto completo
Pereira, Jorge F. [1] ; Araujo, Elza F. [2] ; Brommonschenkel, Sergio H. [3] ; Queiroz, Casley B. [2] ; Costa, Gustavo G. L. [4] ; Carazzolle, Marcelo F. [4] ; Pereira, Goncalo A. G. [4] ; Queiroz, Marisa V. [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Embrapa Trigo, BR-99001970 Passo Fundo, RS - Brazil
[2] Univ Fed Vicosa, Dept Microbiol, BR-36570000 Vicosa, MG - Brazil
[3] Univ Fed Vicosa, Dept Fitopatol, BR-36570000 Vicosa, MG - Brazil
[4] Univ Estadual Campinas, Dept Genet & Evolucao, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: CURRENT GENETICS; v. 61, n. 2, p. 185-202, MAY 2015.
Citações Web of Science: 3

Transposons are an important source of genetic variation. The phytopathogen Moniliophthora perniciosa shows high level of variability but little is known about the role of class I elements in shaping its genome. In this work, we aimed the characterization of a new gypsy/Ty3 retrotransposon species, named MpSaci, in the M. perniciosa genome. These elements are largely variable in size, ranging from 4 to 15 kb, and harbor direct long terminal repeats (LTRs) with varying degrees of similarity. Approximately, all of the copies are non-autonomous as shifts in the reading frame and stop codons were detected. Only two elements (MpSaci6 and MpSaci9) code for GAG and POL proteins that possess functional domains. Conserved domains that are typically not found in retrotransposons were detected and could potentially impact the expression of neighbor genes. Solo LTRs and several LARDs (large retrotransposon derivative) were detected. Unusual elements containing small sequences with or without interruptions that are similar to gag or different pol domains and presenting LTRs with different levels of similarities were identified. Methylation was observed in MpSaci reverse transcriptase sequences. Distribution analysis indicates that MpSaci elements are present in high copy number in the genomes of C-, S- and L-biotypes of M. perniciosa. In addition, C-biotype isolates originating from the state of Bahia have fragments in common with isolates from the Amazon region and two hybridization profiles related to two chromosomal groups. RT-PCR analysis reveals that the gag gene is constitutively expressed and that the expression is increased at least three-fold with nutrient depravation even though no new insertion were observed. These findings point out that MpSaci collaborated and, even though is primarily represented by non-autonomous elements, still might contribute to the generation of genetic variability in the most important cacao pathogen in Brazil. (AU)

Processo FAPESP: 13/08293-7 - CECC - Centro de Engenharia e Ciências Computacionais
Beneficiário:Munir Salomao Skaf
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs