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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Pfaffosidic Fraction from Hebanthe paniculata Induces Cell Cycle Arrest and Caspase-3-Induced Apoptosis in HepG2 Cells

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Autor(es):
da Silva, Tereza Cristina [1] ; Cogliati, Bruno [1] ; Latorre, Andreia Oliveira [1] ; Akisue, Gokithi [2] ; Nagamine, Marcia Kazumi [1] ; Haraguchi, Mitsue [3] ; Hansen, Daiane [3] ; Sanches, Daniel Soares [1] ; Zaidan Dagli, Andmaria Lucia [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, BR-05508900 Sao Paulo, SP - Brazil
[2] Sch Pindamonhangaba, BR-12420010 Pindamonhangaba, SP - Brazil
[3] Biol Inst Sao Paulo, Div Anim Biol, Pharmacol Sect, BR-04014002 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Evidence-based Complementary and Alternative Medicine; 2015.
Citações Web of Science: 0
Resumo

Hebanthe paniculata roots (formerly Pfaffia paniculata and popularly known as Brazilian ginseng) show antineoplastic, chemopreventive, and antiproliferative properties. Functional properties of these roots and their extracts are usually attributed to the pfaffosidic fraction, which is composed mainly by pfaffosides A-F. However, the therapeutic potential of this fraction in cancer cells is not yet entirely understood. This study aimed to analyze the antitumoral effects of the purified pfaffosidic fraction or saponinic fraction on the human hepatocellular carcinoma HepG2 cell line. Cellular viability, proliferation, and apoptosis were evaluated, respectively, by MTT assay, BrdU incorporation, activated caspase-3 immunocytochemistry, and DNA fragmentation assay. Cell cycle was analyzed by flow cytometry and the cell cycle-related proteins were analyzed by quantitative PCR and Western blot. The cells exposed to pfaffosidic fraction had reduced viability and cellular growth, induced G2/M at 48 h or S at 72 h arrest, and increased sub-G1 cell population via cyclin E downregulation, p27(KIP1) overexpression, and caspase-3-induced apoptosis, without affecting the DNA integrity. Antitumoral effects of pfaffosidic fraction from H. paniculata in HepG2 cells originated by multimechanisms of action might be associated with cell cycle arrest in the S phase, by CDK2 and cyclin E downregulation and p27(KIP1) overexpression, besides induction of apoptosis through caspase-3 activation. (AU)

Processo FAPESP: 06/51678-3 - Avaliação dos efeitos de frações purificadas do extrato butanólico das raízes de Pfaffia paniculata sobre o crescimento da linhagem HepG2 de carcinoma hepatocelular humano
Beneficiário:Maria Lucia Zaidan Dagli
Linha de fomento: Auxílio à Pesquisa - Regular