Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Immunohistochemical expression of cyclin D1 is higher in supratentorial ependymomas and predicts relapses in gross total resection cases

Texto completo
Autor(es):
de Andrade, Fernanda Goncalves [1, 2] ; Nagahashi Marie, Suely Kazue [2] ; Uno, Miyuki [2] ; Matushita, Hamilton [1] ; Taricco, Mario Augusto [1] ; Teixeira, Manoel Jacobsen [1] ; Rosemberg, Sergio [3] ; Oba-Shinjo, Sueli Mieko [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Dept Neurol, Div Neurosurg, BR-01246903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Neurol, Cellular & Mol Biol Lab, BR-01246903 Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Hosp Clin, Pathol, BR-01246903 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: NEUROPATHOLOGY; v. 35, n. 4, p. 312-323, AUG 2015.
Citações Web of Science: 2
Resumo

Ependymomas are tumors of the CNS. Although cyclin D1 overexpression has been related to several cancers, its prognostic value in ependymomas has not yet been fully established. We evaluated cyclin D1 expression by an immunohistochemistry analysis of 149 samples of ependymomas, including some relapses, corresponding to 121 patients. Eighty-one patients were adults, 60 were intracranial cases and 92 tumors were grade II. Gross total resection (GTR) was achieved in 62% of cases, and relapse was confirmed in 41.4% of cases. Cyclin D1 protein expression was analyzed by immunohistochemistry and scored with a labeling index (LI) calculated as the percentage of positively stained cells by intensity. We also analyzed expression of CCND1 and NOTCH1 in 33 samples of ependymoma by quantitative real-time PCR. A correlation between cyclin D1 LI score and anaplastic cases (P<0.001), supratentorial location (P<0.001) and age (P=0.001) were observed. A stratified analysis demonstrated that cyclin D1 protein expression was strong in tumors with a supratentorial location, independent of the histological grade or age. Relapse was more frequent in cases with a higher cyclin D1 LI score (P=0.046), and correlation with progression-free survival was observed in cases with GTR (P=0.002). Only spinal canal tumor location and GTR were suggestive markers of PFS in multivarite analyses. Higher expression levels were observed in anaplastic cases for CCND1 (P=0.002), in supratentorial cases for CCND1 (P=0.008) and NOTCH1 (P=0.011). There were correlations between the cyclin D1 mRNA and protein expression levels (P<0.0001) and between CCND1 and NOTCH1 expression levels (P=0.003). Higher cyclin D1 LI was predominant in supratentorial location and predict relapse in GTR cases. Cyclin D1 could be used as an immunohistochemical marker to guide follow-up and treatment in these cases. (AU)

Processo FAPESP: 12/15128-0 - Alterações na expressão gênica dos ependimomas
Beneficiário:Sueli Mieko Oba Shinjo
Linha de fomento: Auxílio à Pesquisa - Regular