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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Structural Characterization of New Peptide Variants Produced by Cyanobacteria from the Brazilian Atlantic Coastal Forest Using Liquid Chromatography Coupled to Quadrupole Time-of-Flight Tandem Mass Spectrometry

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Autor(es):
Sanz, Miriam [1] ; Dini Andreote, Ana Paula [2] ; Fiore, Marli Fatima [2] ; Doerr, Felipe Augusto [1] ; Pinto, Ernani [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Pharmaceut Sci, BL-17055089 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13400970 Piracicaba, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: MARINE DRUGS; v. 13, n. 6, p. 3892-3919, JUN 2015.
Citações Web of Science: 10
Resumo

Cyanobacteria from underexplored and extreme habitats are attracting increasing attention in the search for new bioactive substances. However, cyanobacterial communities from tropical and subtropical regions are still largely unknown, especially with respect to metabolite production. Among the structurally diverse secondary metabolites produced by these organisms, peptides are by far the most frequently described structures. In this work, liquid chromatography/electrospray ionization coupled to high resolution quadrupole time-of-flight tandem mass spectrometry with positive ion detection was applied to study the peptide profile of a group of cyanobacteria isolated from the Southeastern Brazilian coastal forest. A total of 38 peptides belonging to three different families (anabaenopeptins, aeruginosins, and cyanopeptolins) were detected in the extracts. Of the 38 peptides, 37 were detected here for the first time. New structural features were proposed based on mass accuracy data and isotopic patterns derived from full scan and MS/MS spectra. Interestingly, of the 40 surveyed strains only nine were confirmed to be peptide producers; all of these strains belonged to the order Nostocales (three Nostoc sp., two Desmonostoc sp. and four Brasilonema sp.). (AU)

Processo FAPESP: 12/25272-0 - Isolamento e elucidação estrutural de novos compostos com potencial aplicação terapêutica de cianobactérias brasileiras de água doce
Beneficiário:Miriam Sanz Roldán
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/09192-0 - Salinas do Pantanal da Nhecolândia (MS): Hidro-bio-geoquímica de uma área úmida singular
Beneficiário:Laurent Barbiero
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/15402-1 - Cianobactérias da filosfera da Mata Atlântica: isolamento, taxonomia e bioprospecção
Beneficiário:Ana Paula Dini Andreote
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/51950-3 - Prospecting volatile compounds produced by Brazilian cyanobacteria with allelopathic effects
Beneficiário:Ernani Pinto Junior
Linha de fomento: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE