A Metalloproteomics Study on the Association of Mercury With Breast Milk in Samples From Lactating Women in the Amazon Region of Brazil

Autor(es)

dos Santos, Felipe Andre ^[1] ; Cavecci, Bruna ^[2] ; Souza Vieira, Jose Cavalcante ^[2] ; Franzini, Vanessa Pezza ^[3] ; Santos, Ademir ^[3] ; Leite, Aline de Lima ^[4] ; Rabelo Buzalaf, Marilia Afonso ^[4] ; Zara, Luiz Fabricio ^[5] ; Padilha, Pedro de Magalhaes ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ Mato Grosso Sul INQUI UFMS, Inst Chem, Campo Grande, MS - Brazil

^[2] Sao Paulo State Univ IB UNESP, Inst Biosci, Dept Chem & Biochem, BR-18618970 Sao Paulo - Brazil

^[3] Sao Paulo State Univ IB UNESP, Inst Chem, Dept Analyt Chem, BR-18618970 Sao Paulo - Brazil

^[4] Univ Sao Paulo FOB USP, Bauru Dent Sch, Dept Biochem, Sao Paulo - Brazil

^[5] Univ Brasilia UnB, Planaltina, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Archives of Environmental Contamination and Toxicology; v. 69, n. 2, p. 223-229, AUG 2015.
• Citações Web of Science: 8

Resumo

This study aimed to identify metalloproteins that lose their metal ions in the presence of mercury (Hg) and bind to Hg in breast milk samples collected from the riverine population of the Madeira River, a tributary of the Amazon River. Initially, total Hg was determined from the hair of lactating women to identify individuals who were contaminated followed by a proteomic analysis of breast milk samples through two-dimensional polyacrylamide gel electrophoresis after acetone precipitation. The presence of Hg in the obtained protein spots was determined by cold vapor atomic absorption spectrometry and cold vapor atomic fluorescence spectrometry. These determinations indicated the presence of Hg in one protein spot, which was then characterized through electrospray ionization tandem mass spectrometry. Based on searches in the UniProt database, this protein spot was identified as lysozyme C. (AU)

• Processo FAPESP: 13/21297-1 - Metalotioneínas em peixes da região amazônica: estudo metalômico associado ao mercúrio
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51332-5 - Desenvolvimento de métodos analíticos para estudo metalômico do mercúrio em peixes coletados na área de influência do AHE Jirau Bacia do Rio Madeira
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51506-3 - Estudo metalômico do mercúrio em leite materno coletado da população ribeirinha da área de influência do AHE Jirau - Bacia do Rio Madeira
• Beneficiário: Felipe André dos Santos
• Linha de fomento: Bolsas no Brasil - Doutorado