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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Physico-chemical stability and structural characterization of thickened multilamellar beta-carotene-loaded liposome dispersions produced using a proliposome method

Texto completo
Autor(es):
Carvalho, Jose M. P. [1] ; Toniazzo, Taise [1] ; Cavalcanti, Leide P. [2] ; Moraes, Izabel C. F. [1] ; Oliveira, Cristiano L. P. [3] ; Pinho, Samantha C. [1, 4]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Food Engn, Sch Anim Sci & Food Engn, Sao Paulo - Brazil
[2] Brazilian Synchrotron Light Natl Lab, Campinas, SP - Brazil
[3] Univ Sao Paulo, Inst Phys, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Food Engn, Coll Anim Sci & Food Engn, BR-13635900 Pirassununga, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: COLLOID AND POLYMER SCIENCE; v. 293, n. 8, p. 2171-2179, AUG 2015.
Citações Web of Science: 7
Resumo

The objective of this study was to investigate in more detail the structure of multilamellar beta-carotene-loaded liposome dispersions produced by proliposome hydration. Such dispersions were stabilized using xanthan gum as a thickener in different concentrations, and their stabilities were monitored for 90 days. The vesicles exhibited an average diameter in the range of 700 to 3000 nm, and the liposomes were capable of protecting beta-carotene from degradation for a period of 90 days. The dispersions were also characterized by transmission electron microscopy, differential scanning calorimetry, rheology, and small-angle X-ray scattering (SAXS). The thermal analyses showed that neither the beta-carotene nor the xanthan gum affected the liposome bilayer structure. The presence of the xanthan gum, which affects the scattering intensity, was not an obstacle to obtain the structural parameters by SAXS modeling, as a modified modeling strategy (Gaussian deconvolution) was applied. This modeling resulted in 40 symmetric layers, and the results obtained with the variation of temperature were in agreement with the gel-liquid crystalline transition temperature obtained by calorimetric measurements. Additionally, the rheological data showed that xanthan gum was not as effective as a mixture of xanthan gum and guar gum at stabilizing the liposomes, most likely due to the higher stiffness of the gum alone compared with that of its combination with guar gum. (AU)

Processo FAPESP: 11/01277-0 - Estabilização de dispersões lipossomais utilizando hidrocolóides: estudo de caso para encapsulação de beta-caroteno
Beneficiário:Stefany Cho
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 09/01087-7 - Incorporação de carotenóide em lipossomas por hidratação de partículas secas de fosfolipídios
Beneficiário:Samantha Cristina de Pinho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/03901-3 - Dispersões de lipossomas encapsulando beta-caroteno e estabilizadas com hidrocolóides: caracterização e aplicações
Beneficiário:Taíse Toniazzo
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/14868-7 - Estabilização de dispersões de lipossomas através do espessamento com de goma xantana
Beneficiário:Jose Mário Pereira de Carvalho
Linha de fomento: Bolsas no Brasil - Iniciação Científica