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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.

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Autor(es):
Bonifacio, Bruna Vidal [1] ; dos Santos Ramos, Matheus Aparecido [1] ; da Silva, Patricia Bento [2] ; Silveira Negri, Kamila Maria [1] ; Lopes, Erica de Oliveira [1] ; de Souza, Leonardo Perez [3] ; Vilegas, Wagner [4] ; Pavan, Fernando Rogerio [1] ; Chorilli, Marlus [2] ; Bauab, Tais Maria [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Biol Sci, BR-14801902 Araraquara, SP - Brazil
[2] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Drugs & Med, BR-14801902 Araraquara, SP - Brazil
[3] UNESP Univ Estadual Paulista, Inst Chem, Dept Organ Chem, BR-14801902 Araraquara, SP - Brazil
[4] UNESP Univ Estadual Paulista, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 10, p. 5081-5092, 2015.
Citações Web of Science: 13
Resumo

The genus Astronium (Anacardiaceae) includes species, such as Astronium fraxinifolium, Astronium graveolens, and Astronium urundeuva, which possess anti-inflammatory, anti-ulcerogenic, healing, and antimicrobial properties. Nanostructured lipid systems are able to potentiate the action of plant extracts, reducing the required dose and side effects and improving antimicrobial activity. This work aims to evaluate a nanostructured lipid system that was developed as a strategy to improve the anti-Candida albicans activity of hydroethanolic extracts of stems and leaves from Astronium sp. The antifungal activity against C. albicans (ATCC 18804) was evaluated in vitro by a microdilution technique. In addition to the in vitro assays, the Astronium sp. that showed the best antifungal activity and selectivity index was submitted to an in vivo assay using a model of vulvovaginal candidiasis infection. In these assays, the extracts were either used alone or were incorporated into the nanostructured lipid system (comprising 10% oil phase, 10% surfactant, and 80% aqueous phase). The results indicated a minimal inhibitory concentration of 125.00 mu g/mL before incorporation into the nanostructured system; this activity was even more enhanced when this extract presented a minimal inhibitory concentration of 15.62 mu g/mL after its incorporation. In vivo assay dates showed that the nanostructure-incorporated extract of A. urundeuva leaves was more effective than both the unincorporated extract and the antifungal positive control (amphotericin B). These results suggest that this nanostructured lipid system can be used in a strategy to improve the in vitro and in vivo anti-C. albicans activity of hydroethanolic extracts of Astronium sp. (AU)

Processo FAPESP: 09/52237-9 - Fitoterápicos padronizados como alvo para o tratamento de doenças crônicas
Beneficiário:Wagner Vilegas
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
Processo FAPESP: 13/25121-5 - Prospecção químico-biológica de extratos hidroetanólicos das folhas de Astronium urundeuva na investigação do potencial antifúngico
Beneficiário:Bruna Vidal Bonifácio
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/25432-0 - Atividade in vitro e in vivo anti-Candida albicans e anti-Helicobacter pylori de extratos de Astronium sp. incorporados ou não em sistemas nanoestruturados
Beneficiário:Taís Maria Bauab
Linha de fomento: Auxílio à Pesquisa - Regular