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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content

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Autor(es):
Vicentini, Renato [1] ; Bottcher, Alexandra [2] ; Brito, Michael dos Santos [2, 3] ; dos Santos, Adriana Brombini [2] ; Creste, Silvana [3] ; de Andrade Landell, Marcos Guimaraes [3] ; Cesarino, Igor [4] ; Mazzafera, Paulo [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Ctr Mol Biol & Genet Engn, Syst Biol Lab, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil
[3] Agron Inst Campinas, Sugarcane Ctr, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Inst Biosci, Dept Bot, Sao Paulo, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 10, n. 8 AUG 4 2015.
Citações Web of Science: 22
Resumo

Sugarcane is an important crop worldwide for sugar and first generation ethanol production. Recently, the residue of sugarcane mills, named bagasse, has been considered a promising lignocellulosic biomass to produce the second-generation ethanol. Lignin is a major factor limiting the use of bagasse and other plant lignocellulosic materials to produce second-generation ethanol. Lignin biosynthesis pathway is a complex network and changes in the expression of genes of this pathway have in general led to diverse and undesirable impacts on plant structure and physiology. Despite its economic importance, sugarcane genome was still not sequenced. In this study a high-throughput transcriptome evaluation of two sugarcane genotypes contrasting for lignin content was carried out. We generated a set of 85,151 transcripts of sugarcane using RNA-seq and de novo assembling. More than 2,000 transcripts showed differential expression between the genotypes, including several genes involved in the lignin biosynthetic pathway. This information can give valuable knowledge on the lignin biosynthesis and its interactions with other metabolic pathways in the complex sugarcane genome. (AU)

Processo FAPESP: 08/58031-0 - Equilíbrio energético da cana-de-açúcar: uma abordagem de sistemas para compreender a regulação do metabolismo da sacarose e a sinalização de açúcar
Beneficiário:Renato Vicentini dos Santos
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Apoio a Jovens Pesquisadores
Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
Beneficiário:Paulo Mazzafera
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático