Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS

Texto completo
Autor(es):
Mokochinski, Joao Benhur [1] ; Bataglion, Giovana Anceski [2] ; Kiyota, Eduardo [1] ; de Souza, Leticia Marrone [1] ; Mazzafera, Paulo [1] ; Helena Frankland Sawaya, Alexandra Christine [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Dept Plant Biol, Inst Biol, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 407, n. 23, p. 7221-7227, SEP 2015.
Citações Web of Science: 6
Resumo

A simple extraction protocol and an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method for the determination of the syringyl/guaiacyl (S/G) ratio in lignin is reported herein. The method was entirely developed using stems of three Eucalyptus species, which were hydrolyzed with NaOH and partitioned with ethyl ether; vanillin (from the G monomer) and syringaldehyde (from S monomer) were quantified. The S/G ratios obtained were comparable to those usually reported for eucalyptus. The data for one of the eucalyptus species were compared with those obtained with a widely accepted method using thioacidolysis and gas chromatography-mass spectrometry (GC-MS). The method was also applied to sugarcane and showed to be reliable. The yield of the NaOH hydrolysis of the monolignols ranged from 89.94 to 95.69 %, with more than 77.12 % of recuperation in the liquid-liquid extraction. The whole analytical procedure was validated, achieving results with less than 4.38 % of variation. The lowest LOD and LOQ were 0.01 and 0.05 mu g/mL, respectively. In addition, the method combines reliability and a fast and direct quantification. (AU)

Processo FAPESP: 13/21306-0 - Desenvolvimento de métodos analíticos por UPLC-MS para o estudo do metaboloma de duas espécies de eucalipto expostas a variações de temperatura e concentração de CO2
Beneficiário:João Benhur Mokochinski
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/51949-5 - Physiological responses of Eucalyptus globulus and E. grandis to high concentration of CO2 and temperature variations, identified by metabolomic and transcriptomic analyses
Beneficiário:Paulo Mazzafera
Linha de fomento: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE
Processo FAPESP: 12/21395-0 - Caracterização molecular da matéria orgânica sedimentar do Sistema Estuarino-Lagunar de Cananeia-Iguape por espectrometria de massas de ressonânica ciclotrônica de íons com Transformada de Fourier
Beneficiário:Giovana Anceski Bataglion
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
Beneficiário:Paulo Mazzafera
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático