Lipidomic fatty acid profile and global gene expression pattern in mammary gland of rats that were exposed to lard-based high fat diet during fetal and lactation periods associated to breast cancer risk in adulthood

Autor(es)

Andrade, Fabia de Oliveira ^[1] ; de Assis, Sonia ^[2] ; Jin, Lu ^[2] ; Fontelles, Camile Castilho ^[1] ; Barbisan, Luis Fernando ^[3] ; Purgatto, Eduardo ^[1] ; Hilakivi-Clarke, Leena ^[2] ; Ong, Thomas Prates ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, BR-05508900 Sao Paulo, SP - Brazil

^[2] Georgetown Univ, Sch Med, Lombardi Comprehens Canc Ctr, Dept Oncol, Washington, DC 20007 - USA

^[3] State Univ Sao Paulo Botucatu, Botucatu Biosci Inst, Dept Morphol, BR-18618000 Botucatu, SP - Brazil

^[4] Food Res Ctr NAPAN, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemico-Biological Interactions; v. 239, p. 118-128, SEP 5 2015.
• Citações Web of Science: 7

Resumo

The persistent effects of animal fat consumption during pregnancy and nursing on the programming of breast cancer risk among female offspring were studied here. We have previously found that female offspring of rat dams that consumed a lard-based high-fat (HF) diet (60% fat-derived energy) during pregnancy, or during pregnancy and lactation, were at a reduced risk of developing mammary cancer. To better understand the unexpected protective effects of early life lard exposure, we have applied lipidomics and nutrigenomics approaches to investigate the fatty acid profile and global gene expression patterns in the mammary tissue of the female offspring. Consumption of this HF diet during gestation had few effects on the mammary tissue fatty acids profile of young adult offspring, while exposure from gestation throughout nursing promoted significant alterations in the fatty acids profile. Major differences were related to decreases in saturated fatty acids (SFA) and increases in omega-6 polyunsaturated fatty acids (PUFAs), monounsaturated fatty acids (MUFAs) and conjugated linolenic acid (CIA) concentrations. In addition several differences in gene expression patterns by microarray analysis between the control and in utero or in utero and during lactation HF exposed offspring were identified. Differential dependency network (DDN) analysis indicated that many of the genes exhibited unique connections to other genes only in the HF offspring. These unique connections included Hrhl-Ythdf1 and Repinl-Elavl2 in the in utero HF offspring, and Rnf213-Htr3b and Klf5-Chrna4 in the in utero and lactation HF offspring, compared with the control offspring. We conclude that an exposure to a lard-based HF diet during early life changes the fatty acid profile and transcriptional network in mammary gland in young adult rats, and these changes appear to be consistent with reduced mammary cancer risk observed in our previous study. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/03330-9 - Análise da morfologia e expressão gênica global de tecido mamário de ratas tratadas in útero com ração rica em ácidos graxos saturados
• Beneficiário: Fábia de Oliveira Andrade
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 10/11742-0 - Nutrição e origem fetal do câncer de mama: consumo de ração rica em gordura saturada por ratas durante a gestação e suscetibilidade da prole feminina à carcinogênese mamária
• Beneficiário: Fábia de Oliveira Andrade
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/23259-4 - Nutrição materna e programação fetal do câncer de mama: importância da interação nutriente-epigenoma nas fases iniciais da vida
• Beneficiário: Thomas Prates Ong
• Modalidade de apoio: Auxílio à Pesquisa - Regular