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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Gene expression patterns through oral squamous cell carcinoma development: PD-L1 expression in primary tumor and circulating tumor cells

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Autor(es):
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Oliveira-Costa, Joao Paulo [1] ; de Carvalho, Alex Fiorini [2] ; da Silveira, Giorgia Gobbi [1] ; Amaya, Peter [3] ; Wu, Yongqi [3] ; Park, Kyoung-Joo Jenny [3] ; Gigliola, Mabel Pinilla [2] ; Lustberg, Maryam [4] ; Cavicchioli Buim, Marcilei Eliza [5] ; Ferreira, Elisa Napolitano [2] ; Kowalski, Luiz Paulo [6] ; Chalmers, Jeffrey J. [3] ; Soares, Fernando Augusto [5] ; Carraro, Dirce Maria [2] ; Ribeiro-Silva, Alfredo [1]
Número total de Autores: 15
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Pathol, BR-14049 Ribeirao Preto - Brazil
[2] AC Camargo Canc Ctr, CIPE, Lab Genom & Mol Biol, Sao Paulo - Brazil
[3] Ohio State Univ, William G Lowrie Dept Chem & Biomol Engn, Columbus, OH 43210 - USA
[4] Ohio State Univ, Wexner Med Ctr, Stefanie Spielman Comprehens Breast Ctr, Columbus, OH 43210 - USA
[5] AC Camargo Canc Ctr, Dept Anat Pathol, Sao Paulo - Brazil
[6] AC Camargo Canc Ctr, Dept Head & Neck Surg & Otorhinolaryngol, Sao Paulo - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: ONCOTARGET; v. 6, n. 25, p. 20902-20920, AUG 28 2015.
Citações Web of Science: 46
Resumo

Oral squamous cell carcinoma (OSCC) is the most common tumor of the oral cavity and has been associated with poor prognosis. Scarce prognostic markers are available for guiding treatment and/or sub-classifying patients. This study aims to identify biomarkers by searching for genes whose expression is increased or decreased during tumor progression (through T1 to T4 stages). Thirty-six samples from all tumor size stages (from T1 to T4) were analyzed using cDNA microarrays. Selected targets were analyzed by immunohistochemistry and in circulating tumor cells by immunofluorescence and Nanostring. Correlation was shown between PD-L1 and tumor size and lymph node metastasis, HOXB9 and tumor size, BLNK and perineural invasion, and between ZNF813 and perineural invasion. PD-L1 positivity was an independent prognostic factor in this cohort (p = 0.044, HH = 0.426). In CTCs from patients with locally advanced OSCC, we found a strong cytoplasmatic expression of PD-L1. PD-L1 is a ligand of PD-1 and is believed to limit T cell activity in inflammatory responses and limit autoimmune diseases. We demonstrated an important role for PD-L1 in primary tumors according to tumor size, and in disease specific survival. Therefore, we could further determine individuals with PD-L1+ CTCs, and possibly follow treatment using CTCs. (AU)

Processo FAPESP: 14/14991-1 - Avaliação do status dos receptores do fator de crescimento de fibroblastos (FGF) em carcinomas epidermóides e em células tumorais circulantes
Beneficiário:João Paulo Oliveira da Costa
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/08637-0 - Identificação de padrões moleculares relacionados ao desenvolvimento do carcinoma epidermóide oral utilizando a tecnologia de micro-arranjos de cDNA
Beneficiário:Alfredo Ribeiro da Silva
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/18606-7 - CDNA e lincRNA microarrays: identificação de padrões de expressão gênica relacionados à metastatização em carcinomas epidermóides orais
Beneficiário:Fernando Augusto Soares
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/08400-0 - Identificação de padrões moleculares relacionados ao desenvolvimento do carcinoma epidermóide oral utilizando a tecnologia de micro-arranjos de cDNA
Beneficiário:João Paulo Oliveira da Costa
Linha de fomento: Bolsas no Brasil - Doutorado