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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effective protection of biological membranes against photo-oxidative damage: Polymeric antioxidant forming a protecting shield over the membrane

Texto completo
Autor(es):
Mertins, Omar [1, 2] ; Mathews, Patrick D. [1] ; Gomide, Andreza B. [2] ; Baptista, Mauricio S. [3] ; Itri, Rosangela [2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Dept Biophys, Escola Paulista Med, BR-04023062 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Phys, Dept Appl Phys, BR-05508900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1848, n. 10, A, p. 2180-2187, OCT 2015.
Citações Web of Science: 7
Resumo

We have prepared a chitosan polymer modified with gallic acid in order to develop an efficient protection strategy biological membranes against photodamage. Lipid bilayers were challenged with photoinduced damage by photosensitization with methylene blue, which usually causes formation of hydroperoxides, increasing area per lipid, and afterwards allowing leakage of internal materials. The damage was delayed by a solution of gallic acid in a concentration dependent manner, but further suppressed by the polymer at very low concentrations. The membrane of giant unilamellar vesicles was covered with this modified macromolecule leading to a powerful shield against singlet oxygen and thus effectively protecting the lipid membrane from oxidative stress. The results have proven the discovery of a promising strategy for photo protection of biological membranes. (C) 2015 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 10/18163-5 - Foto-proteção em membranas investigada pela técnica de microscopia óptica em vesículas unilamelares gigantes
Beneficiário:Omar Mertins
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 12/50680-5 - Fotossensibilização nas ciências da vida
Beneficiário:Mauricio da Silva Baptista
Linha de fomento: Auxílio à Pesquisa - Temático