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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Self-Assembled Arginine-Capped Peptide Bolaamphiphile Nanosheets for Cell Culture and Controlled Wettability Surfaces

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Autor(es):
da Silva, Emerson Rodrigo [1, 2] ; Walter, Merlin Nathaniel Mark [3] ; Reza, Mehedi [4] ; Castelletto, Valeria [2] ; Ruokolainen, Janne [4] ; Connon, Che John [3] ; Alves, Wendel Andrade [1] ; Hamley, Ian William [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre - Brazil
[2] Univ Reading, Dept Chem, Reading RG6 6AD, Berks - England
[3] Newcastle Univ, Inst Med Genet, Int Ctr Life, Newcastle Upon Tyne NE1 3BZ, Tyne & Wear - England
[4] Aalto Univ, Sch Sci, Dept Appl Phys, FI-00076 Aalto - Finland
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Biomacromolecules; v. 16, n. 10, p. 3180-3190, OCT 2015.
Citações Web of Science: 23
Resumo

The spontaneous assembly of a peptide bolaamphiphile in water, namely, RFL4FR (R, arginine; F, phenylalanine; L, leucine) is investigated, along with its novel properties in surface modification and usage as substrates for cell culture. RFL4FR self-assembles into nanosheets through lateral association of the peptide backbone. The 1,4 sequence is located within the core of the nanosheets, whereas the R moieties are exposed to the water at the surface of the nanosheets. Kinetic assays indicate that the self-assembly is driven by a remarkable two-step process, where a nucleation phase is followed by fast growth of nanosheets with an autocatalysis process. The internal structure of the nanosheets is formed from ultrathin bolaamphiphile monolayers with a crystalline orthorhombic symmetry with cross-beta organization. We show that human corneal stromal fibroblast (hCSF) cells can grow on polystyrene films coated with films dried from RFL4FR solutions. For the first time, this type of amphiphilic peptide is used as a substrate to modulate the wettability of solid surfaces for cell culture applications. (AU)

Processo FAPESP: 14/03514-8 - Investigações estruturais em sistemas-modelo baseados em peptídeos anfifílicos
Beneficiário:Emerson Rodrigo da Silva
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
Processo FAPESP: 13/12997-0 - Auto-organização hierárquica de peptídeos anfifílicos: mecanismos fundamentais e potenciais aplicações
Beneficiário:Wendel Andrade Alves
Linha de fomento: Auxílio à Pesquisa - Regular