Selenium fractionation from plasma, muscle and liver of Nile tilapia (Oreochromis niloticus)

Autor(es)

Silva, Fabio A. ^[1] ; Cavecci, Bruna ^[1] ; Baldassini, Welder A. ^[1] ; Lima, Paula M. ^[1] ; Moraes, Paula M. ^[2] ; Roldan, Paulo S. ^[3] ; Padilha, Cilene C. F. ^[2] ; Padilha, Pedro M. ^{[2, 4]}

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Fac Vet Med & Anim Sci, Botucatu, SP - Brazil

^[2] UNESP Sao Paulo State Univ, Bioci Inst, Botucatu, SP - Brazil

^[3] Fed Univ Alagoas IQB UFAL, Inst Chem & Biotechnol, Maceio, AL - Brazil

^[4] Univ Estadual Campinas, Natl Inst Bioanalyt Sci & Technol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION; v. 7, n. 4, p. 158-165, DEC 2013.
• Citações Web of Science: 4

Resumo

This paper presents the results obtained from selenium fractionation in plasma, muscle and liver samples of Nile tilapia's (Oreochromis niloticus) after protein separation. The plasma, muscle and liver proteome was obtained by 2D-PAGE, and selenium in protein spots was qualitatively and quantitatively determined by synchrotron radiation X-ray fluorescence and graphite furnace atomic absorption spectrometry (GFAAS). The fluorescence spectra indicated the presence of selenium in three protein spots of plasma, two of muscle and one of liver. Selenium was found to be distributed mainly in proteins with a molar mass smaller than 57.0 kDa and with pI in the range of 5.9-9.6, with one exception in the plasma sample, which presented protein with a molar mass of 60.0 kDa. After acid mineralization of the protein spots, a GFAAS determination of the concentration of selenium bound to these proteins indicated a range of 1.35-6.82 mg per g of protein. (AU)

• Processo FAPESP: 06/55039-5 - Avaliacao de salenoproteinas de tilapia do nilo utilizando-se espectrometria de absorcao atomica em forno de grafite e eletroforese em gel.
• Beneficiário: Fábio Arlindo Silva
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/59778-0 - Desenvolvimento de metodologias analíticas para avaliação de metaloproteínas de tilápia do Nilo (Oreochromis niloticus)
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular