POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells

Autor(es)

Franca, M. M. ^[1] ; Abreu, N. P. ^[1] ; Vrechi, T. A. M. ^[1] ; Lotfi, C. F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, BR-05508 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 48, n. 12, p. 1087-1094, DEC 2015.
• Citações Web of Science: 6

Resumo

During gonad and adrenal development, the POD-1/capsulin/TCF21transcription factor negatively regulates SF-1/NR5A1expression, with higher SF-1 levels being associated with increased adrenal cell proliferation and tumorigenesis. In adrenocortical tumor cells, POD-1 binds to the SF-1 E-box promoter region, decreasing SF-1 expression. However, the modulation of SF-1 expression by POD-1 has not previously been described in normal adrenal cells. Here, we analyzed the basal expression of Pod-1 and Sf-1 in primary cultures of glomerulosa (G) and fasciculata/reticularis (F/R) cells isolated from male Sprague-Dawley rats, and investigated whether POD-1 overexpression modulates the expression of endogenous Sf-1 and its target genes in these cells. POD-1 overexpression, following the transfection of pCMVMycPod-1, significantly decreased the endogenous levels of Sf-1 mRNA and protein in F/R cells, but not in G cells, and also decreased the expression of the SF-1 target StAR in F/R cells. In G cells overexpressing POD-1, no modulation of the expression of SF-1 targets, StAR and CYP11B2, was observed. Our data showing that G and F/R cells respond differently to ectopic POD-1 expression emphasize the functional differences between the outer and inner zones of the adrenal cortex, and support the hypothesis that SF-1 is regulated by POD-1/Tcf21 in normal adrenocortical cells lacking the alterations in cellular physiology found in tumor cells. (AU)

• Processo FAPESP: 12/19512-9 - Análise das vias de sinalização induzidas por peptídeos NH2-terminal da POMC (N-POMC) em culturas primárias de suprarrenal de rato
• Beneficiário: Talita Aparecida de Moraes Vrechi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/00771-9 - O papel e os mecanismos de regulação dos fatores de transcrição Sf-1 e Lrh-1 em culturas de células de tumores da suprarrenal humana
• Beneficiário: Monica Malheiros França
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/21839-6 - Estudo do ciclo celular das células de tumores adrenocorticais: análise da expressão de oncogenes e genes supressores de tumores, e da proliferação através da ação de quimioterápicos
• Beneficiário: Claudimara Ferini Pacicco Lotfi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/01031-1 - Estudo da expressão do fator de transcrição SF-1 e sua regulação em culturas primárias de adrenal de rato: o papel de Pod-1/TCF-21
• Beneficiário: Nayara Pereira de Abreu
• Modalidade de apoio: Bolsas no Brasil - Mestrado