In Vitro Identification of Histatin 5 Salivary Complexes

Autor(es)

Moffa, Eduardo B. ^{[1, 2, 3, 4]} ; Machado, Maria A. A. M. ^[2] ; Mussi, Maria C. M. ^{[1, 5]} ; Xiao, Yizhi ^[1] ; Garrido, Saulo S. ^[6] ; Giampaolo, Eunice T. ^[3] ; Siqueira, Walter L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Western Ontario, Dept Dent & Biochem, Schulich Sch Med & Dent, London, ON - Canada

^[2] Univ Sao Paulo, Bauru Dent Sch, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, SP - Brazil

^[3] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Sao Paulo - Brazil

^[4] CEUMA Univ, Dept Prosthodont, Sao Luis, MA - Brazil

^[5] Univ Sao Paulo, Sch Dent, Sao Paulo, SP - Brazil

^[6] UNESP Univ Estadual Paulista, Inst Chem, Dept Biochem & Technol Chem, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 11 NOV 6 2015.
• Citações Web of Science: 7

Resumo

With recent progress in the analysis of the salivary proteome, the number of salivary proteins identified has increased dramatically. However, the physiological functions of many of the newly discovered proteins remain unclear. Closely related to the study of a protein's function is the identification of its interaction partners. Although in saliva some proteins may act primarily as single monomeric units, a significant percentage of all salivary proteins, if not the majority, appear to act in complexes with partners to execute their diverse functions. Coimmunoprecipitation (Co-IP) and pull-down assays were used to identify the heterotypic complexes between histatin 5, a potent natural antifungal protein, and other salivary proteins in saliva. Classical protein-protein interaction methods in combination with high-throughput mass spectrometric techniques were carried out. Co-IP using protein G magnetic Sepharose TM beads suspension was able to capture salivary complexes formed between histatin 5 and its salivary protein partners. Pull-down assay was used to confirm histatin 5 protein partners. A total of 52 different proteins were identified to interact with histatin 5. The present study used proteomic approaches in conjunction with classical biochemical methods to investigate protein-protein interaction in human saliva. Our study demonstrated that when histatin 5 is complexed with salivary amylase, one of the 52 proteins identified as a histatin 5 partner, the antifungal activity of histatin 5 is reduced. We expected that our proteomic approach could serve as a basis for future studies on the mechanism and structural-characterization of those salivary protein interactions to understand their clinical significance. (AU)

• Processo FAPESP: 12/01528-6 - Efetividade de vernizes fotopolimerizáveis experimentais e da escovação na redução da adesão e formação de biofilmes em uma resina para base de prótese
• Beneficiário: Eunice Teresinha Giampaolo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/23540-5 - Ação da histatina-5, um peptídeo de desinfecção, sobre células planctônicas e biofilme de Candida albicans
• Beneficiário: Eunice Teresinha Giampaolo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/23543-4 - "Ação da Histatina-5, um peptídeo de desinfecção, sobre células planctônicas e biofilme de Candida Albicans."
• Beneficiário: Eduardo Buozi Moffa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/15412-2 - Análise por de espectrometria de massas dos complexos salivares formados com a histatina-5
• Beneficiário: Eduardo Buozi Moffa
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado