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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Glutathione modifies the oxidation products of 2 `-deoxyguanosine by singlet molecular oxygen

Texto completo
Autor(es):
Peres, Patricia S. [1] ; Valerio, Andressa [1] ; Cadena, Silvia M. S. C. [1] ; Winnischofer, Sheila M. B. [1] ; Scalfo, Alexsandra C. [2] ; Di Mascio, Paolo [2] ; Martinez, Glaucia R. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Parana, Setor Ciencias Biol, Dept Bioquim & Biol Mol, Lab Oxidacoes Biol, BR-80060000 Curitiba, PR - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo, PR - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Archives of Biochemistry and Biophysics; v. 586, p. 33-44, NOV 15 2015.
Citações Web of Science: 4
Resumo

The oxidation of the free nucleoside 2'-deoxyguanosine (dGuo) by singlet molecular oxygen (O-1(2)) has been studied over the three last decades due to the major role of DNA oxidation products in process such as ageing, mutation and carcinogenesis. In the present work we investigated the dGuo oxidation by O-1(2) in the presence of the important low molecular antioxidant, glutathione, in its reduced (GSH) and oxidized (GSSG) forms. There were applied different conditions of concentration, pH, time of incubation, and the use of a {[}O-18]-labeled thermolabile endoperoxide naphthalene derivative as a source of {[}O-18]labeled O-1(2). Data was obtained through high performance liquid chromatography (HPLC) and HPLC coupled to micrOTOF Q-II analysis of the main oxidation products: the diastereomers of spiroiminodihydantoin-2'-deoxyribonucleosides (dSp) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo). An intriguing result was that 8-oxodGuo levels increased by 100 fold when dGuo was oxidized by O-1(2) in the presence of GSH and by 2 fold in the presence of GSSG, while dSp levels dropped to zero for both conditions. All data from dGuo, 8-oxodGuo and dSp quantification together with the analysis of residual GSH/GSSG content in each sample strongly suggest that glutathione modifies the mechanism of dGuo oxidation by O-1(2) by disfavoring the pathway of dSp formation. (C) 2015 Published by Elsevier Inc. (AU)

Processo FAPESP: 12/12663-1 - Oxigênio singlete e peróxidos em química biológica
Beneficiário:Paolo Di Mascio
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs