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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effects of low-level laser therapy on the expression of osteogenic genes during the initial stages of bone healing in rats: a microarray analysis

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Autor(es):
Tim, Carla Roberta [1] ; Bossini, Paulo Sergio [2] ; Kido, Hueliton Wilian [1] ; Malavazi, Iran [3] ; von Zeska Kress, Marcia Regina [4] ; Carazzolle, Marcelo Falsarella [5, 6] ; Parizotto, Nivaldo Antonio [1] ; Renn, Ana Claudia [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Physiotherapy, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biosci, BR-11050240 Santos - Brazil
[3] Univ Fed Sao Carlos, Dept Genet & Evolut, BR-13565905 Sao Carlos, SP - Brazil
[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Bromatol, BR-14049900 Ribeirao Preto - Brazil
[5] Univ Estadual Campinas, Dept Genet & Evolut, BR-13083970 Campinas, SP - Brazil
[6] Brazilian Natl Ctr Res Energy & Mat, Brazilian Biosci Natl Lab, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Lasers in Medical Science; v. 30, n. 9, p. 2325-2333, DEC 2015.
Citações Web of Science: 11
Resumo

This study evaluated the morphological changes produced by LLLT on the initial stages of bone healing and also studied the pathways that stimulate the expression of genes related to bone cell proliferation and differentiation. One hundred Wistar rats were divided into control and treated groups. Noncritical size bone defects were surgically created at the upper third of the tibia. Laser irradiation (Ga-Al-As laser 830 nm, 30 mW, 94 s, 2.8 J) was performed for 1, 2, 3, 5, and 7 sessions. Histopathology revealed that treated animals produced increased amount of newly formed bone at the site of the injury. Moreover, microarray analysis evidenced that LLLT produced a significant increase in the expression TGF-beta, BMP, FGF, and RUNX-2 that could stimulate osteoblast proliferation and differentiation, which may be related to improving the deposition of newly formed bone at the site of the injury. Thus, it is possible to conclude that LLLT improves bone healing by producing a significant increase in the expression of osteogenic genes. (AU)

Processo FAPESP: 10/15335-0 - Análise da expressão gênica durante o reparo ósseo em defeitos tíbias irradiados com laser terapêutico de baixa intensidade
Beneficiário:Carla Roberta Tim
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/08293-7 - CECC - Centro de Engenharia e Ciências Computacionais
Beneficiário:Munir Salomao Skaf
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs