Salt-induced sympathoexcitation involves vasopressin V-1a receptor activation in the paraventricular nucleus of the hypothalamus

Autor(es)

Ribeiro, Natalia ^[1] ; Panizza, Helena do Nascimento ^[1] ; dos Santos, Karoline Martins ^[1] ; Ferreira-Neto, Hildebrando C. ^[1] ; Antunes, Vagner Roberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY; v. 309, n. 11, p. R1369-R1379, DEC 1 2015.
• Citações Web of Science: 10

Resumo

A high-salt diet can lead to hydromineral imbalance and increases in plasma sodium and osmolality. It is recognized as one of the major contributing factors for cardiovascular diseases such as hypertension. The paraventricular nucleus (PVN) plays a pivotal role in osmotically driven sympathoexcitation and high blood pressure, the precise mechanisms of which are not fully understood. Recent evidence indicates that AVP released from magnocellular neurons might be involved in this process. Using a combination of in vivo and in situ studies, we sought to investigate whether AVP, acting on PVN neurons, can change mean arterial pressure (MAP) and sympathetic nerve activity (SNA) in euhydrated male rats. Furthermore, we wanted to determine whether V-1a receptors on PVN neurons would be involved in salt-induced sympathoexcitation and hypertension. In rats, 4 days of salt loading (NaCl 2%) elicited a significant increase in plasma osmolality (39 +/- 7 mosmol/kgH(2)O), an increase in MAP (26 +/- 2 mmHg, P < 0.001), and sympathoexcitation compared with euhydrated rats. Microinjection of AVP into the PVN of conscious euhydrated animals (100 nl, 3 mu M) elicited a pressor response (14 +/- 2 mmHg) and a significant increase in lumbar SNA (100 nl, 1 mM) (19 +/- 5%). Pretreatment with a V-1a receptor antagonist, microinjected bilaterally into the PVN of salt-loaded animals, elicited a decrease in lumbar SNA (-14 +/- 5%) and MAP (-19 +/- 5 mmHg), when compared with the euhydrated group. Our findings show that AVP plays an important role in modulating the salt-induced sympathoexcitation and high blood pressure, via V-1a receptors, within the PVN of male rats. As such, V-1a receptors in the PVN might contribute to neurogenic hypertension in individuals consuming a high-salt diet. (AU)

• Processo FAPESP: 10/17997-0 - Mecanismos da neurotransmissão purinérgica do hipotálamo sobre a atividade autonômica simpática e sua relação com a hipertensão neurogênica
• Beneficiário: Vagner Roberto Antunes
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/05037-1 - Papel dos receptores purinérgicos do núcleo paraventricular do hipotálamo na hipertensão neurogênica
• Beneficiário: Hildebrando Candido Ferreira Neto
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/13047-0 - Papel do sistema nervoso autonômico sobre a produção hepática de glicose via ação central de insulina em animais hipertensos
• Beneficiário: Izabela Martina Ramos Ribeiro de Toledo
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/06206-0 - Estudo da expressão gênica de neuropeptídeos e mediadores inflamatórios em núcleos do eixo hipotálamo-tronco cerebral envolvidos no controle neural da pressão arterial de animais submetidos a dieta obesogênica
• Beneficiário: Vagner Roberto Antunes
• Linha de fomento: Auxílio à Pesquisa - Regular