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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Type 1 cannabinoid receptor modulates water deprivation-induced homeostatic responses

Texto completo
Autor(es):
Ruginsk, Silvia G. [1, 2] ; Vechiato, Fernanda M. V. [1] ; Uchoa, Ernane T. [3, 1] ; Elias, Lucila L. K. [1] ; Antunes-Rodrigues, Jose [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Physiol, Sao Paulo - Brazil
[2] Univ Fed Alfenas, Inst Biomed Sci, Dept Physiol Sci, Alfenas, MG - Brazil
[3] Univ Estadual Londrina, Dept Physiol Sci, Londrina, Parana - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY; v. 309, n. 11, p. R1358-R1368, DEC 1 2015.
Citações Web of Science: 5
Resumo

The present study investigated the type 1 cannabinoid receptor (CB1R) as a potential candidate to mediate the homeostatic responses triggered by 24 h of water deprivation, which constitutes primarily a hydroelectrolytic challenge and also significantly impacts energy homeostasis. The present results demonstrated for the first time that CB1R mRNA expression is increased in the hypothalamus of water-deprived (WD) rats. Furthermore, the administration of ACEA, a CB1R selective agonist, potentiated WD-induced dipsogenic effect, whereas AM251, a CB1R antagonist, attenuated not only water but also salt intake in response to WD. In parallel with the modulation of thirst and salt appetite, we confirmed that CB(1)Rs are essential for the development of appropriated neuroendocrine responses. Although the administration of ACEA or AM251 did not produce any effects on WD-induced arginine vasopressin (AVP) secretion, oxytocin (OXT) plasma concentrations were significantly decreased in WD rats treated with ACEA. At the genomic level, ACEA significantly decreased AVP and OXT mRNA expression in the hypothalamus of WD rats, whereas AM251 potentiated both basal and WD-induced stimulatory effects on the transcription of AVP and OXT genes. In addition, we showed that water deprivation alone upregulated proopiomelanocortin, Agouti-related peptide, melanin-concentrating hormone, and orexin A mRNA levels in the hypothalamus, and that CB1Rs regulate main central peptidergic pathways controlling food intake, being that most of these effects were also significantly influenced by the hydration status. In conclusion, the present study demonstrated that CB(1)Rs participate in the homeostatic responses regulating fluid balance and energy homeostasis during water deprivation. (AU)

Processo FAPESP: 10/16692-0 - Avaliação da participação do receptor canabinóide do tipo CB1 nas respostas neuroendócrinas e sua possível interação com o sistema angiotensinérgico no modelo de privação hídrica
Beneficiário:Fernanda Maria Veanholi Vechiato
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 08/50611-8 - Regulação neuroendócrina do equilíbrio hidrossalino e da ingestão alimentar: aspectos fisiológicos, fisiopatológicos e moleculares
Beneficiário:José Antunes Rodrigues
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 10/02748-4 - O apetite por água e sódio no modelo de privação hídrica: modulação pelo sistema endocanabinóide
Beneficiário:Silvia Graciela Ruginsk Leitão
Linha de fomento: Bolsas no Brasil - Pós-Doutorado