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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization of Leptospira interrogans serovar Pomona isolated from swine in Brazil

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Autor(es):
Miraglia, Fabiana [1] ; Moreno, Luisa Zanolli [1] ; Morais, Zenaide Maria [1] ; Langoni, Helio [2] ; Shimabukuro, Fabio Hiroto [3] ; Dellagostin, Odir Antonio [4] ; Hartskeerl, Rudy [5, 6] ; Vasconcellos, Silvio Arruda [1] ; Moreno, Andrea Micke [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508 Sao Paulo - Brazil
[2] UNESP, Lab Diagnost Zoonoses, Botucatu, SP - Brazil
[3] Adolfo Lutz Inst, Lab Reg Sorocaba, Sorocaba - Brazil
[4] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol Biotecnol, Pelotas - Brazil
[5] Royal Trop Inst, KIT Biomed Res, Natl Leptospirosis Reference Ctr, NL-1105 AZ Amsterdam - Netherlands
[6] Royal Trop Inst, KIT Biomed Res, WHO FAO OIE, NL-1105 AZ Amsterdam - Netherlands
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF INFECTION IN DEVELOPING COUNTRIES; v. 9, n. 10, p. 1054-1061, OCT 2015.
Citações Web of Science: 5
Resumo

Introduction: Leptospira interrogans swine infection is a cause of serious economic loss and a potential human health hazard. In Brazil, the most common serovars associated with swine infections are Pomona, Icterohaemorrhagie and Tarassovi. Cross-reactions among serovars and the failure of infected animals to seroconvert may complicate the interpretation of serological tests. Molecular methods with better discriminatory powers are useful tools for swine leptospirosis characterization and diagnosis. Methodology: This study evaluated nine L. interrogans isolates from the States of Sao Paulo and Minas Gerais during different time periods. Isolates from diseased and apparently healthy swine were characterized by microscopic agglutination tests with polyclonal antibodies and were genotyped by VNTR, PFGE and MLST techniques. Broth microdilution was used to determine the minimal inhibitory concentration of the antimicrobials of veterinary interest. Results: The strains were identified as L. interrogans serogroup Pomona serovar Pomona Genotype A, while MLST grouped all of the isolates in sequence type 37. The PFGE analysis resulted in two pulsotypes with more than 70% similarity, distinguishing serovar Pomona isolates from the serovar Kennewicki reference strain. All of the isolates presented low MIC values to penicillin, ampicillin, ceftiofur and tulathromycin. High MIC values for fluoroquinolones, tiamulin, gentamicin, tetracyclines, neomycin, tilmicosin and sulfas were also observed. Conclusions: All molecular techniques were concordant in L. interrogans serovar Pomona identification. This serovar may have a different antibiotic susceptibility profile than previously reported for Leptospira isolates. (AU)

Processo FAPESP: 13/17136-2 - Caracterização de estirpes de Leptospira isoladas no Brasil através de tipificação por eletroforese em campo pulsado (PFGE) e análise comparativa de genomas
Beneficiário:Luisa Zanolli Moreno
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 09/52925-2 - Estudo comparativo de procedimentos destinados ao controle da potência de bacterinas anti-leptospirose suína
Beneficiário:Silvio Arruda Vasconcellos
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/18290-0 - Caracterização de estirpes de Leptospira isoladas no Brasil através da tipagem por sequenciamento de multilocus (MLST)
Beneficiário:Andrea Micke Moreno
Linha de fomento: Auxílio à Pesquisa - Regular