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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of the local inflammatory events induced by BpirMP, a metalloproteinase from Bothrops pirajai venom

Texto completo
Autor(es):
Bernardes, Carolina P. [1] ; Menaldo, Danilo L. [1] ; Mamede, Carla C. N. [2, 3] ; Zoccal, Karina F. [1] ; Cintra, Adelia C. O. [1] ; Faccioli, Lucia H. [1] ; Stanziola, Leonilda [2, 3] ; de Oliveira, Fabio [2, 3] ; Sampaio, Suely V. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Fed Uberlandia, ICBIM, Inst Ciencias Biomed, BR-38400 Uberlandia, MG - Brazil
[3] Inst Natl Ciencia & Tecnol Nanobiofarmaceut N Bio, Belo Horizonte, MG - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Molecular Immunology; v. 68, n. 2, B, p. 456-464, 2015.
Citações Web of Science: 11
Resumo

In this study, we evaluated the edema and hyperalgesic response induced by BpirMP, a P-I class metalloproteinase isolated from Bothrops pirajai snake venom. The animals were injected with the metalloproteinase or sterile PBS (control group) and evaluated for 1, 2, 3, 4, 5, 6 and 24h. The intraplantar injection of BpirMP (5-50 mu g/paw) induced a dose- and time-dependent response. BpirMP (50 mu g) induced paw edema in rats rapidly, with peak response two hours after injection of the toxin. Also, BpirMP injection caused a significant reduction in the nociceptive threshold of the animals tested, with peak response three hours after injection of the toxin. The inflammatory mediators involved in these responses were assayed by pretreatment of animals with synthesis inhibitors or receptor antagonists. Peak responses were significantly reduced by pretreatment of animals with pyrilamine, a histamine receptor antagonist, sodium cromoglycate, a mast cell degranulation inhibitor and valeryl salicylate and meloxicam, cyclooxygenase inhibitors. The analysis of the peritoneal cavity exudate revealed an acute inflammatory response with recruitment of leukocytes, increased levels of total proteins, nitric oxide and the cytokines IL-6, TNF-alpha and IL-10. In conclusion, our results demonstrated that BpirMP induces inflammation mediated by mast cell degranulation, histamine, prostaglandins and cytokine production. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 07/08361-1 - Caracterização funcional e estrutural de uma metaloprotease não-hemorrágica com atividades antitumoral e microbicida isolada do veneno de Bothrops pirajai
Beneficiário:Carolina Petri Bernardes
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
Beneficiário:Suely Vilela
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 05/54855-0 - Toxinas animais: estrutura, função e aplicações biotecnológicas
Beneficiário:Suely Vilela
Linha de fomento: Auxílio à Pesquisa - Temático