Enterotoxigenic and non-enterotoxigenic Bacteroides fragilis from fecal microbiota of children

Autor(es)

Ignacio, Aline ^[1] ; Fernandes, Miriam Rodriguez ^[1] ; Avila-Campos, Mario Julio ^[1] ; Nakano, Viviane ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 46, n. 4, p. 1141-1145, OCT-DEC 2015.
• Citações Web of Science: 3

Resumo

Enterotoxigenic Bacteroides fragilis (ETBF) is an important part of the human and animal intestinal microbiota and is commonly associated with diarrhea. ETBF strains produce an enterotoxin encoded by the bft gene located in the B. fragilis pathogenicity island (BfPAI). Non-enterotoxigenic B. fragilis (NTBF) strains lack the BfPAI and usually show two different genetic patterns, II and III, based on the absence or presence of a BfPAI-flanking region, respectively. The incidence of ETBF and NTBF strains in fecal samples isolated from children without acute diarrhea or any other intestinal disorders was determined. All 84 fecal samples evaluated were B. fragilis-positive by PCR, four of them harbored the bft gene, 27 contained the NTBF pattern III DNA sequence, and 52 were considered to be NTBF pattern II samples. One sample was positive for both ETBF and NTBF pattern III DNA sequences. All 19 B. fragilis strains isolated by the culture method were bft-negative, 9 belonged to pattern III and 10 to pattern II. We present an updated overview of the ETBF and NTBF incidence in the fecal microbiota of children from Sao Paulo City, Brazil. (AU)

• Processo FAPESP: 12/10659-7 - Avaliação de alguns microrganismos da microbiota intestinal endógena de crianças eutróficas, obesas e com sobrepeso em idade escolar
• Beneficiário: Aline Ignacio Silvestre da Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/03792-0 - Expressão dos genes bmeABC de bombas de efluxo de Bacteroides fragilis em Escherichia coli, caracterização do perfil de substrato das bombas bmeABC e sua relação com a resistência aos antimicrobianos
• Beneficiário: Viviane Nakano
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 08/57330-4 - Expressão dos genes bmeABC de bombas de efluxo de Bacteroides fragilis em Escherichia coli, caracterização do perfil de substrato das bombas bmeABC e sua relação com a resistência aos antimicrobianos
• Beneficiário: Viviane Nakano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores