Ultrasound-assisted encapsulation of annatto seed oil: Retention and release of a bioactive compound with functional activities

Autor(es)

Silva, Eric Keven ^[1] ; Zabot, Giovani L. ^[1] ; Meireles, M. Angela A. ^[1]

Afiliação do(s) autor(es)

^[1] UNICAMP Univ Campinas, LASEFI DEA FEA Sch Food Engn, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 78, p. 159-168, DEC 2015.
• Citações Web of Science: 27

Resumo

This paper brings forward the encapsulation of annatto seed oil (rich in geranylgeraniol) assisted by high intensity ultrasound using gum Arabic (GA) as stabilizing agent. We studied the effects of time (min) and ultrasonication power (W) over the emulsion characteristics. After forming microparticles from the best emulsion using freeze-drying (FD) and spray-drying (SD) techniques, we evaluated particle size distribution, moisture, water activity, surface oil, entrapment efficiency, encapsulation efficiency, geranylgeraniol retention, oxidative stability and kinetic release of geranylgeraniol, a biocompound with functional activities. The combined intensification of time and ultrasonication power reduced the superficial mean diameter (D-32) and polydispersity (PDI) of emulsions. Drying the continuous phase of the optimized emulsion (smallest D-32 = 0.69 +/- 0.03 mu m) using FD and SD formed microparticles with different morphological characteristics, Brouckere diameter (D-43), particle size distribution, moisture and water activity. SD process led to microparticles with the highest oil encapsulation efficiency (85.1 +/- 0.1 wt.%) as a consequence of their lowest surface oil (SO). However, GA-FD microparticles presented the highest oil entrapment efficiency (97 +/- 1 wt.%). Geranylgeraniol retention (80-86 wt.%) was similar for both drying techniques. GA-FD microparticles were more stable against oxidation through accelerated test Rancimat, even though presenting higher SO. This behavior is associated with the likely phase transition on the GA-SD matrix. The difference on the kinetic release of geranylgeraniol is linked to the difference on the particles morphology and particle size distribution. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/15685-1 - Intensificação de processos para obtenção de extratos naturais e formação de partículas on-line usando tecnologia supercrítica
• Beneficiário: Giovani Leone Zabot
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/10685-8 - Combinação/acoplamento de processos para a produção e encapsulamento de extratos vegetais de alto valor agregado: avaliação técnica e econômica
• Beneficiário: Maria Angela de Almeida Meireles Petenate
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático