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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

An Intracellular Arrangement of Histoplasma capsulaturn Yeast-Aggregates Generates Nuclear Damage to the Cultured Murine Alveolar Macrophages

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Autor(es):
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Pitangui, Nayla de Souza [1] ; Sardi, Janaina de Cassia Orlandi [1] ; Voltan, Aline R. [1] ; dos Santos, Claudia T. [1] ; da Silva, Julhiany de Fatima [1] ; da Silva, Rosangela A. M. [1] ; Souza, Felipe O. [1] ; Soares, Christiane P. [1] ; Rodriguez-Arellanes, Gabriela [2] ; Lucia Taylor, Maria [2] ; Mendes-Giannini, Maria J. S. [1] ; Fusco-Almeida, Ana M. [1]
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara - Brazil
[2] Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF - Mexico
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN MICROBIOLOGY; v. 6, JAN 11 2016.
Citações Web of Science: 0
Resumo

Histoplasma capsulatum is responsible for a human systemic mycosis that primarily affects lung tissue. Macrophages are the major effector cells in humans that respond to the fungus, and the development of respiratory disease depends on the ability of Histoplasma yeast cells to survive and replicate within alveolar macrophages. Therefore, the interaction between macrophages and H. capsulatum is a decisive step in the yeast dissemination into host tissues. Although the role played by components of cell-mediated immunity in the host's defense system and the mechanisms used by the pathogen to evade the host immune response are well understood, knowledge regarding the effects induced by H. capsulatum in host cells at the nuclear level is limited. According to the present findings, H. capsulaturr yeast cells display a unique architectural arrangement during the intracellular infection of cultured murine alveolar macrophages, characterized as a formation of aggregates that seem to surround the host cell nucleus, resembling a ``crown.{''} This extranuclear organization of yeast-aggregates generates damage on the nucleus of the host cell, producing DNA fragmentation and inducing apoptosis, even though the yeast cells are not located inside the nucleus and do not trigger changes in nuclear proteins. The current study highlights a singular intracellular arrangement of H. capsulaturr yeast near to the nucleus of infected murine alveolar macrophages that may contribute to the yeast's persistence under intracellular conditions, since this fungal pathogen may display different strategies to prevent elimination by the host's phagocytic mechanisms. (AU)

Processo FAPESP: 13/05853-1 - Análise proteômica e transcriptômica do biofilme de Histoplasma capsulatum e correlação com fatores de virulência na busca de novos alvos terapêuticos
Beneficiário:Ana Marisa Fusco Almeida
Linha de fomento: Auxílio à Pesquisa - Regular