Nanoparticles Based on Chitosan as Carriers for the Combined Herbicides Imazapic and Imazapyr

Autor(es)

Maruyama, Cintia Rodrigues ^{[1, 2, 3]} ; Guilger, Mariana ^{[2, 3]} ; Pascoli, Monica ^{[1, 2, 3]} ; Bileshy-Jose, Natalia ^[2] ; Abhilash, P. C. ^[4] ; Fraceto, Leonardo Fernandes ^[1] ; de Lima, Renata ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Environm Engn, Sorocaba - Brazil

^[2] Univ Sorocaba, Biotechnol Lab, Km 92, BR-18023000 Sorocaba - Brazil

^[3] Univ Fed Sao Carlos, Sorocaba Campus, Km 110, BR-18052780 Sorocaba - Brazil

^[4] Banaras Hindu Univ, Inst Environm & Sustainable Dev, Varanasi 221005, Uttar Pradesh - India

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, JAN 27 2016.
• Citações Web of Science: 33

Resumo

The use of lower concentrations and fewer applications of herbicides is one of the prime objectives of the sustainable agriculture as it decreases the toxicity to non-targeted organisms and the risk of wider environmental contamination. In the present work, nanoparticles were developed for encapsulation of the herbicides imazapic and imazapyr. Alginate/chitosan and chitosan/tripolyphosphate nanoparticles were manufactured, and their physicochemical stability was evaluated. Determinations were made of the encapsulation efficiency and release kinetics, and the toxicity of the nanoparticles was evaluated using cytotoxicity and genotoxicity assays. The effects of herbicides and herbicide-loaded nanoparticles on soil microorganisms were studied in detail using real-time polymerase chain reactions. The nanoparticles showed an average size of 400 nm and remained stable during 30 days of storage at ambient temperature. Satisfactory encapsulation efficiencies of between 50 and 70% were achieved for both types of particles. Cytotoxicity assays showed that the encapsulated herbicides were less toxic, compared to the free compounds, and genotoxicity was decreased. Analyses of soil microbiota revealed changes in the bacteria of the soils exposed to the different treatments. Our study proves that encapsulation of the herbicides improved their mode of action and reduced their toxicity, indicating their suitability for use in future practical applications. (AU)

• Processo FAPESP: 14/21618-5 - Avaliação da toxicidade em organismos aquático (Danio rerio) e microbiota do solo expostos a nanopartículas metálicas (TiO2, Ag-NP e Fe-NP) utilizando marcadores genéticos e bioquímicos
• Beneficiário: Renata de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular