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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Baccharis dracunculifolia-based mouthrinse alters the exopolysaccharide structure in cariogenic biofilms

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Autor(es):
Aires, Carolina P. [1] ; Sassaki, Guilherme L. [2] ; Santana-Filho, Arquimedes P. [2] ; Spadaro, Augusto C. C. [1] ; Cury, Jaime A. [3]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Chem & Phys, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Fed Parana, Dept Biochem & Mol Biol, POB 19046, BR-81531980 Curitiba, Parana - Brazil
[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Ave Limeira 901, BR-13414903 Piracicaba, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Macromolecules; v. 84, p. 301-307, MAR 2016.
Citações Web of Science: 1
Resumo

Baccharis dracunculifolia is a native plant from Brazil with antimicrobial activity. The purpose of this study was to investigate whether a B. dracunculifolia-based mouthrinse (Bd) changes the structure of insoluble exopolysaccharides (IEPS) in Streptococcus mutans UA159 cariogenic biofilm. Biofilms were grown on glass slides and treated with Bd, its vehicle (VC), chlorhexidine digluconate (CHX), or saline solution (NaCl). Among the treatments, only CHX significantly reduced the biofilm biomass and bacterial viability (p < 0.05). Gas chromatography mass spectrometry and nuclear magnetic resonance analyses revealed that IEPS from the four biofilm samples were alpha-glucans containing different proportions of (1 6) and (I 3) glycosidic linkages. The structural differences among the four IEPS were compared by principal component analysis (PCA). PCA analysis indicated that IEPS from VC- and NaCl-treated biofilms were structurally similar to each other. Compared with the control, IEPS from Bd- and CHX-treated biofilms were structurally different and had distinct chemical profiles. In summary, the fact that Bd changed the IEPS chemical composition indicates that this mouthrinse may affect the cariogenic properties of the S. mutans biofilm formed. (C) 2015 Published by Elsevier B.V. (AU)

Processo FAPESP: 10/12456-0 - Efeito da sacarose e de antimicrobiano natural na estrutura química de polissacarídeos do biofilme dental
Beneficiário:Carolina Patrícia Aires Garbellini
Linha de fomento: Auxílio à Pesquisa - Regular