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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of the sensitivity of IgG and IgM ELISA in detecting Schistosoma mansoni infections in a low endemicity setting

Texto completo
Autor(es):
Espirito-Santo, M. C. C. [1] ; Sanchez, M. C. A. [1] ; Sanchez, A. R. [1] ; Alvarado-Mora, M. V. [1] ; Castilho, V. L. P. [1] ; Goncalves, E. M. N. [1] ; Luna, E. J. A. [1] ; Gryschek, R. C. B. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES; v. 33, n. 12, p. 2275-2284, DEC 2014.
Citações Web of Science: 8
Resumo

Schistosomiasis is a major public health concern, with 200 million people infected worldwide. In Brazil, this disease has been reported in 19 states, and its prevalence in the city of Barra Mansa in Rio de Janeiro State is 1 %. The parasitological diagnostic methods currently available in these areas lack sensitivity; however, enzyme-linked immunosorbent assays (ELISAs) have been employed successfully for the diagnosis of schistosomiasis by using antibodies against antigens of Schistosoma mansoni adult worms and eggs, and for the detection of circulating antigens. The objective of this study was to determine systematically the prevalence of S. mansoni infection in the peripheral areas of Barra Mansa. A cross-sectional study was conducted from April to December 2011 by using probabilistic sampling that collected 610 fecal samples and 612 serum samples. ELISA-IgG with total extracts and ELISA-IgM with trichloroacetic acid-soluble fractions were employed to detect antibodies against S. mansoni and were compared with the Kato-Katz and Hoffman parasitological techniques. Among the individuals studied, anti-S. mansoni antibodies were detected in 11.16 % (n = 71) by ELISA-IgG and in 20.75 % (n = 132) by ELISA-IgM, while the parasitological techniques showed 0.82 % (n = 5) positivity. The agreement between the two ELISA tests was 85.38 % (n = 543), and 8.65 % (n = 55) of the serum samples showed positive results in both tests. The higher positivity of the ELISA-IgM test corroborates the results of previous reports and indicates that the test may be a useful tool in epidemiological studies, particularly in areas of low endemicity for S. mansoni. (AU)

Processo FAPESP: 07/53457-7 - Estudo da variabilidade genotípica dos vírus das Hepatites B e C na Colômbia
Beneficiário:João Renato Rebello Pinho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
Beneficiário:Monica Viviana Alvarado Mora
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 10/52615-0 - Estudo comparativo de métodos diagnósticos da esquistossomose mansoni em áreas de baixa transmissão e prevalência
Beneficiário:Ronaldo Cesar Borges Gryschek
Linha de fomento: Auxílio à Pesquisa - Regular