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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil

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Autor(es):
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Carvalho Espirito-Santo, Maria Cristina [1, 2, 3] ; Alvarado-Mora, Monica Viviana [4, 5] ; Silva Pinto, Pedro Luiz [6] ; Arroyo Sanchez, Maria Carmen [7] ; Dias-Neto, Emmanuel [8, 9] ; Pagliusi Castilho, Vera Lucia [10] ; do Nascimento Goncalves, Elenice Messias [10] ; Chieffi, Pedro Paulo [11, 12] ; de Albuquerque Luna, Expedito Jose [12] ; Rebello Pinho, Joao Renato [4, 5] ; Carrilho, Flair Jose [1, 2] ; Borges Gryschek, Ronaldo Cesar [1, 2]
Número total de Autores: 12
Afiliação do(s) autor(es):
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[1] Univ Sao Paulo, Dept Infect & Parasit Dis, Sch Med, BR-05403000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Lab Immunopathol Schistosomiasis LIM 06, Sch Med, BR-05403000 Sao Paulo, SP - Brazil
[3] Univ Ctr Volta Redonda, BR-27240560 Volta Redonda, RJ - Brazil
[4] Univ Sao Paulo, Dept Gastroenterol, Sch Med, BR-05403000 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Lab Trop Gastroenterol & Hepatol, Sch Med, BR-05403000 Sao Paulo, SP - Brazil
[6] Adolfo Lutz Inst, Dept Enteroparasites, Parasitol & Mycol Serv, BR-01246902 Sao Paulo - Brazil
[7] Univ Sao Paulo, Lab Seroepidemiol & Immunobiol, Inst Trop Med, BR-05403000 Sao Paulo, SP - Brazil
[8] AC Camargo Canc Ctr, Lab Med Genom, BR-01509010 Sao Paulo, SP - Brazil
[9] Univ Sao Paulo, Inst Psychiat LIM 27, Sao Paulo Med Sch, BR-01246903 Sao Paulo, SP - Brazil
[10] Univ Sao Paulo, Sect Parasitol, Cent Lab Div Hosp Clin, Sch Med, BR-05403000 Sao Paulo, SP - Brazil
[11] Santa Casa Med Sch, BR-01221020 Sao Paulo, SP - Brazil
[12] Univ Sao Paulo, Inst Trop Med, BR-05403000 Sao Paulo, SP - Brazil
Número total de Afiliações: 12
Tipo de documento: Artigo Científico
Fonte: BIOMED RESEARCH INTERNATIONAL; 2015.
Citações Web of Science: 7
Resumo

Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay-(ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM(21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82-95.5%), differed significantly from COPT in positivity (P < 0.05), and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs. (AU)

Processo FAPESP: 07/53457-7 - Estudo da variabilidade genotípica dos vírus das Hepatites B e C na Colômbia
Beneficiário:João Renato Rebello Pinho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/52615-0 - Estudo comparativo de métodos diagnósticos da esquistossomose mansoni em áreas de baixa transmissão e prevalência
Beneficiário:Ronaldo Cesar Borges Gryschek
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
Beneficiário:Monica Viviana Alvarado Mora
Linha de fomento: Bolsas no Brasil - Doutorado Direto