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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Anti-inflammatory and antinociceptive effects of racemic goniothalamin, a styryl lactone

Texto completo
Autor(es):
Vendramini-Costa, Debora Barbosa [1, 2] ; Spindola, Humberto Moreira [2] ; de Mello, Glaucia Coelho [3] ; Antunes, Edson [3] ; Pilli, Ronaldo Aloise [1] ; de Carvalho, Joao Ernesto [2, 4]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Dept Organ Chem, Inst Chem, BR-13081970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, CPQBA, Chem Biol & Agr Pluridisciplinary Res Ctr, BR-13148218 Sao Paulo - Brazil
[3] Univ Estadual Campinas, Dept Pharmacol, Fac Med Sci, Cidade Univ Zeferino Vaz, BR-13081970 Campinas, SP - Brazil
[4] Univ Estadual Campinas, Fac Pharmaceut Sci, Cidade Univ Zeferino Vaz, BR-13081970 Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Life Sciences; v. 139, p. 83-90, OCT 15 2015.
Citações Web of Science: 14
Resumo

Aims: The present study aimed to further investigate the anti-inflammatory activity of goniothalamin (GTN), a styryl lactone, as well as its antinociceptive effects. Main methods: The anti-inflammatory activity was evaluated in models of paw edema induced by different mediators in mice and carrageenan-induced peritonitis. Evaluation of the antinociceptive effect was performed through acetic acid-induced writhing test and formalin test. Activity of GTN on gene expression levels of interleukin-1beta (IL-1 beta), induced nitric oxidase synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated in vitro in lipopolysaccharide (LPS)-stimulated macrophage (RAW 264.7), as well as gene expression and protein levels of tumor necrosis factor-alpha (TNF-alpha). Key findings: Pretreatment with GIN (300 mg/kg) significantly reduced paw edema induced by compound 48/80, prostaglandin E-2, phospholipase A(2) and bradyldnin. GIN (10,30 and 100 mg/kg) inhibited leukocyte migration in the peritonitis model and gene expression levels of IL-1 beta, iNOS and TNF-alpha, as well as TNF-alpha protein levels, in LPS-stimulated macrophages, without affecting COX-2 gene expression levels. GIN inhibited nociception induced by acetic acid in the writhing model and in the formalin test, when both neurogenic and inflammatory phases were inhibited. Significance: For the first time the acute anti-inflammatory profile of GTN is characterized and its antinociceptive activity reported. The current study shows that GTN inhibits both vascular and cellular phases of inflammation, with bradykinin and PLA(2) induced inflammation being the most affected by GIN. Its anti-inflammatory effects also involved the in vitro inhibition of gene expression of alarm cytokines and mediators as IL-1 beta, iNOS and TNF-alpha. (C) 2015 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 12/18281-3 - Mecanismo de ação da goniotalamina e derivados: uso de sondas fluorescentes e receptores de folato
Beneficiário:Débora Barbosa Vendramini Costa
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 09/51602-5 - Biologia química: novos alvos moleculares naturais e sintéticos contra o câncer, estudos estruturais, avaliação biológica e modo de ação
Beneficiário:Ronaldo Aloise Pilli
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 07/58665-7 - Atividade anticâncer e mecanismo de morte celular de derivados de stiril-lactonas
Beneficiário:Débora Barbosa Vendramini Costa
Linha de fomento: Bolsas no Brasil - Doutorado Direto