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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In Vitro Regulation of CCL3 and CXCL12 by Bacterial By-products Is Dependent on Site of Origin of Human Oral Fibroblasts

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Autor(es):
Sipert, Carla Renata [1] ; Morandini, Ana Carolina [1] ; Dionisio, Thiago Jose [1] ; Andrade Moreira Machado, Maria Aparecida [2] ; Penha Oliveira, Sandra Helena [3] ; Campanelli, Ana Paula [1] ; Kuo, Winston Patrick [4, 5] ; Santos, Carlos Ferreira [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Sao Paulo - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Community Hlth, Sao Paulo - Brazil
[3] Univ Estadual Paulista, Aracatuba Sch Dent, Dept Basic Sci, Sao Paulo - Brazil
[4] Harvard Univ, Sch Dent Med, Dept Dev Biol, Boston, MA 02115 - USA
[5] Harvard Univ, Sch Med, Harvard Clin & Translat Sci Ctr, Lab Innovat Translat Technol, Boston, MA - USA
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF ENDODONTICS; v. 40, n. 1, p. 95-100, JAN 2014.
Citações Web of Science: 9
Resumo

Introduction: Production of chemokines by tissue resident cells is one of the main mechanisms involved in the inflammatory infiltrate formation during inflammation. The specific ability of fibroblasts from different oral tissues such as gingiva, periodontal ligament, and dental pulp from permanent and deciduous teeth in producing the chennokines CCL3 and CXCL12 under stimulation by bacterial products commonly found in endodontic infections was investigated. Methods: Cultures of fibroblasts from gingiva and periodontal ligament as well as from dental pulp from permanent and deciduous teeth were established by using an explant technique and stimulated with increasing concentrations of Escherichia coli lipopolysaccharide (EcLPS) and Enterococcus faecalis lipoteichoic acid (EfLTA) for 1, 6, and 24 hours. Supernatants were tested for CCL3 and CXCL12 by enzyme-linked immunosorbent assay. Results: In general, CCL3 production was induced by EcLPS in the 4 fibroblast subtypes and by EfLTA in fibroblasts from gingiva and periodontal ligament Constitutive CXCL12 synthesis decreased in all fibroblast subtypes especially under stimulation with EcLPS. Fibroblast from permanent deciduous teeth was the cell type presenting the most expressive reduction in CXCL12 release by both stimuli. On the basis of computational matching of CXCL12 mRNA with the microRNAs miR-141 and miR-200a, their expression was also investigated. Although detected in the fibroblasts, these molecules remained unaltered by bacterial by-product stimulation. Conclusions: EcLPS and EfLTA induced the production of CCL3 and unbalanced the synthesis of CXCL12 in a manner dependent on the specific tissue origin of fibroblasts. (AU)

Processo FAPESP: 09/53848-1 - EMU: Aquisição de dois equipamentos de grande porte (Milliplex Analyzer Xponent 3 e acessórios e 7900 HT Fast Real Time PCR System e acessórios) para a realização de pesquisas por pesquisadores de diversos departamentos da FOB-USP e de outras instituições de pesquisa
Beneficiário:Carlos Ferreira dos Santos
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 05/60167-0 - Expressão de pró-colágeno tipo 1, MIP-1± alfa e SDF-1 alfa por fibroblastos da polpa humana estimulados por ácido lipoteicóico de Streptococcus mutans
Beneficiário:Carlos Ferreira dos Santos
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/00306-1 - Expressão de pró-colágeno do tipo I, MIP-1alfa e SDF-1alfa por fibroblastos de gengiva e polpa dental humanas estimulados por ácido lipoteicóico de Enterococcus faecalis e lipopolissacarídeo de Escherichia coli e Porphyromonas gingivalis
Beneficiário:Carla Renata Sipert
Linha de fomento: Bolsas no Brasil - Doutorado