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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Treatment with triterpenic fraction purified from Baccharis uncinella leaves inhibits Leishmania (Leishmania) amazonensis spreading and improves Th1 immune response in infected mice

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Autor(es):
Yamamoto, Eduardo Seiji [1] ; Campos, Bruno Luiz S. [1] ; Laurenti, Marcia Dalastra [1] ; Lago, Joao H. G. [2] ; Grecco, Simone dos Santos [3] ; Corbett, Carlos E. P. [1] ; Passero, Luiz Felipe D. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med, Dept Patol, Lab Patol Molestias Infeciosas, BR-01246000 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Sao Paulo - Brazil
[3] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210180 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Parasitology Research; v. 113, n. 1, p. 333-339, JAN 2014.
Citações Web of Science: 16
Resumo

The current medications used to treat leishmaniasis have many side effects for patients; in addition, some cases of the disease are refractory to treatment. Therefore, the search for new leishmanicidal compounds is indispensable. Recently, it was demonstrated that oleanolic- and ursolic-containing fraction from Baccharis uncinella leaves eliminated the promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and L. (Viannia) braziliensis without causing toxic effects for J774 macrophages. Thus, the aim of the present work was to characterize the therapeutic effect of the triterpenic fraction in L. (L.) amazonensis-infected BALB/c mice. Oleanolic- and ursolic acid-containing fraction was extracted from B. uncinella leaves using organic solvents and chromatographic procedures. L. (L.) amazonensis-infected BALB/c mice were treated intraperitoneally with triterpenic fraction during five consecutive days with 1.0 and 5.0 mg/kg of triterpenic fraction, or with 10.0 mg/kg of amphotericin B drug. Groups of mice treated with the triterpenic fraction, presented with decreased lesion size and low parasitism of the skin-both of which were associated with high amounts of interleukin-12 and interferon gamma. The curative effect of this fraction was similar to amphotericin B-treated mice; however, the final dose, required to eliminate amastigotes, was lesser than amphotericin B. Moreover, triterpenic fraction did not cause microscopic alterations in liver, spleen, heart, lung, and kidney of experimental groups. This work suggests that this fraction possesses compounds that are characterized by leishmanicidal and immunomodulatory activities. From this perspective, the triterpenic fraction can be explored as a new therapeutic agent for use against American Tegumentar Leishmaniasis. (AU)

Processo FAPESP: 12/03903-9 - Análise da ação dos ácidos oleanólico e ursólico purificados de Baccharis uncinella em Camundongos Balb/c infectados com Leishmania (Leishmania) amazonensis
Beneficiário:Eduardo Seiji Yamamoto
Linha de fomento: Bolsas no Brasil - Iniciação Científica