Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Influence of Candida krusei and Candida glabrata on Candida albicans gene expression in in vitro biofilms

Texto completo
Autor(es):
Barros, Patricia Pimentel [1] ; Ribeiro, Felipe Camargo [1] ; Rossoni, Rodnei Dennis [1] ; Junqueira, Juliana Campos [1] ; Cardoso Jorge, Antonio Olavo [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ORAL BIOLOGY; v. 64, p. 92-101, APR 2016.
Citações Web of Science: 15
Resumo

Objective: The present study aimed to evaluate the interactions between the species Candida albicans, Candida krusei and Candida glabrata in monotypic and mixed biofilm models formed in vitro as well as the relative expression of the ALS1, ALS3, HWP1, BCR1, EFG1, TEC1, SAP5, PLB2 and LIP9 genes. Material and methods: Mixed (C. albicans/C. krusei and C albicans/C. glabrata) and monotypic biofilms were cultured for 0, 12 and 24 h. Gene expression was analyzed in the same biofilm model in which the number of CFU/mL was counted. Results: The C albicans CFU/mL values were lower at the 12 and 24 h time points in the mixed biofilms compared with the monotypic biofilms, and decreases of 56.23% and 64.4% in C albicans were observed when this species was associated with C glabrata and C krusei, respectively. In the presence of C krusei, the expression of the ALS3, HWP1, BCR1, EFG1 and TEC1 genes of C albicans was completely inhibited, indicating both transcriptome and the phenotypic antagonism between these two species, but genes related to the secretion of enzymes were stimulated. In the presence of C glabrata, C albicans showed a similar gene expression profile to that obtained in association with C. krusei, though it was altered to a lesser degree. Conclusion: We conclude that C. krusei and C glabrata may alter or inhibit the mechanisms involved in the in vitro adherence and formation of C albicans biofilms, influencing the pathogenicity of this species and suggesting a competitive interaction with C krusei and C glabrata in biofilm formation. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 11/15194-0 - Interações entre diferentes Espécies de Candida na candidose experimental em modelos de invertebrados e vertebrados
Beneficiário:Rodnei Dennis Rossoni
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 12/15250-0 - Avaliação temporal da expressão dos genes SAP5, SAP9, PLB1, PLB2, LIP9 e LIP10 em interações entre espécies de c. albicans, c. tropicalis, c. glabrata e c. krusei
Beneficiário:Patricia Pimentel de Barros
Linha de fomento: Bolsas no Brasil - Doutorado