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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Xylella fastidiosa outer membrane vesicles modulate plant colonization by blocking attachment to surfaces

Texto completo
Autor(es):
Ionescu, Michael [1] ; Zaini, Paulo A. [2] ; Baccari, Clelia [1] ; Tran, Sophia [1] ; da Silva, Aline M. [2] ; Lindow, Steven E. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 - USA
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Proceedings of the National Academy of Sciences of the United States of America; v. 111, n. 37, p. E3910-E3918, SEP 16 2014.
Citações Web of Science: 40
Resumo

Outer membrane vesicles (OMVs) of Gram-negative bacteria have been studied intensively in recent years, primarily in their role in delivering virulence factors and antigens during pathogenesis. However, the near ubiquity of their production suggests that they may play other roles, such as responding to envelope stress or trafficking various cargoes to prevent dilution or degradation by other bacterial species. Here we show that OMVs produced by Xylella fastidiosa, a xylem-colonizing plant pathogenic bacterium, block its interaction with various surfaces such as the walls of xylem vessels in host plants. The release of OMVs was suppressed by the diffusible signal factor-dependent quorum-sensing system, and a X. fastidiosa Delta rpfF mutant in which quorum signaling was disrupted was both much more virulent to plants and less adhesive to glass and plant surfaces than the WT strain. The higher virulence of the Delta rpfF mutant was associated with fivefold higher numbers of OMVs recovered from xylem sap of infected plants. The frequency of attachment of X. fastidiosa to xylem vessels was 20-fold lower in the presence of OMVs than in their absence. OMV production thus is a strategy used by X. fastidiosa cells to adjust attachment to surfaces in its transition from adhesive cells capable of insect transmission to an ``exploratory{''} lifestyle for systemic spread within the plant host which would be hindered by attachment. OMV production may contribute to the movement of other bacteria in porous environments by similarly reducing their contact with environmental constituents. (AU)

Processo FAPESP: 11/09409-3 - Tecnologia microfluídica para estudo dos mecanismos de adesão de bactérias fitopatogênicas
Beneficiário:Paulo Adriano Zaini
Linha de fomento: Bolsas no Brasil - Pós-Doutorado