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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Streptococcus mutans Can Modulate Biofilm Formation and Attenuate the Virulence of Candida albicans

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Autor(es):
Barbosa, Junia Oliveira [1] ; Rossoni, Rodnei Dennis [1] ; Godinho Vilela, Simone Furgeri [1] ; de Alvarenga, Janana Araujo [1] ; Velloso, Marisol dos Santos [1] ; de Azevedo Prata, Marcia Cristina [2] ; Cardoso Jorge, Antonio Olavo [1] ; Junqueira, Juliana Campos [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil
[2] Embrapa Gado Leite, Juiz De Fora, MG - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 11, n. 3 MAR 2 2016.
Citações Web of Science: 20
Resumo

Streptococcus mutans and Candida albicans are found together in the oral biofilms on dental surfaces, but little is known about the ecological interactions between these species. Here, we studied the effects of S. mutans UA159 on the growth and pathogencity of C. albicans. Initially, the effects of S. mutans on the biofilm formation and morphogenesis of C. albicans were tested in vitro. Next, we investigate the influence of S. mutans on pathogenicity of C. albicans using in vivo host models, in which the experimental candidiasis was induced in G. mellonella larvae and analyzed by survival curves, C. albicans count in hemolymph, and quantification of hyphae in the host tissues. In all the tests, we evaluated the direct effects of S. mutans cells, as well as the indirect effects of the subproducts secreted by this microorganism using a bacterial culture filtrate. The in vitro analysis showed that S. mutans cells favored biofilm formation by C. albicans. However, a reduction in biofilm viable cells and inhibition of hyphal growth was observed when C. albicans was in contact with the S. mutans culture filtrate. In the in vivo study, injection of S. mutans cells or S. mutans culture filtrate into G. mellonella larvae infected with C. albicans increased the survival of these animals. Furthermore, a reduction in hyphal formation was observed in larval tissues when C. albicans was associated with S. mutans culture filtrate. These findings suggest that S. mutans can secrete subproducts capable to inhibit the biofilm formation, morphogenesis and pathogenicity of C. albicans, attenuating the experimental candidiasis in G. mellonella model. (AU)

Processo FAPESP: 12/15262-8 - Interação entre Streptococcus mutans e Candida albicans: formação de biofilme in vitro e estudo experimental em modelos de invertebrados
Beneficiário:Júnia Oliveira Barbosa
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 12/19915-6 - Interação entre Streptococcus mutans e Lactobacillus acidophilus com Candida albicans: formação de biofilme in vitro e estudo experimental em modelos de invertebrados
Beneficiário:Juliana Campos Junqueira
Linha de fomento: Auxílio à Pesquisa - Regular