Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status

Autor(es):Mostrar menos -

dos Santos Hamilton, Thais Rose ^[1] ; de Castro, Leticia Signori ^[1] ; Delgado, Juliana de Carvalho ^[1] ; de Assis, Patricia Monken ^[1] ; Perez Siqueira, Adriano Felipe ^[1] ; Mendes, Camilla Mota ^[1] ; Goissis, Marcelo Demarchi ^[1] ; Muino-Blanco, Teresa ^[2] ; Cebrian-Perez, Jose Alvaro ^[2] ; Nichi, Marcilio ^[1] ; Visintin, Jose Antonio ^[1] ; Ortiz D'Avila Assumpcao, Mayra Elena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Ave Prof Dr Orlando Marques Paiva 87, Cidade Univ, BR-05508270 Sao Paulo - Brazil

^[2] Univ Zaragoza, Dept Biochem & Mol & Cell Biol, Inst Invest Ciencias Ambientales Aragon IUCA, Grp Biol & Fisiol Reprod, Fac Vet, Miguel Servet 177, Zaragoza 50013 - Spain

• Tipo de documento: Artigo Científico
• Fonte: Reproduction; v. 151, n. 4, p. 379-390, APR 2016.
• Citações Web of Science: 14

Resumo

Action of reactive oxygen species, protamination failures and apoptosis are considered the most important etiologies of sperm DNA fragmentation. This study evaluated the effects of induced lipid peroxidation susceptibility on native semen profile and identified the mechanisms involved in sperm DNA fragmentation and testicular antioxidant defense on Santa Ines ram sperm samples. Semen was collected from 12 adult rams (Ovis aries) performed weekly over a 9-week period. Sperm analysis (motility, mass motility, abnormalities, membrane and acrosome status, mitochondrial potential, DNA fragmentation, lipid peroxidation and intracellular free radicals production); protamine deficiency; PRM1, TNP1 and TNP2 gene expression; and determination of glutathione peroxidase (GPx), glutathione reductase, catalase (CAT) and superoxide dismutase activity and immunodetection in seminal plasma were performed. Samples were distributed into four groups according to the sperm susceptibility to lipid peroxidation after induction with ascorbate and ferrous sulfate (low, medium, high and very high). The results were analyzed by GLM test and post hoc least significant difference. We observed an increase in native GPx activity and CAT immunodetection in groups with high susceptibility to induced lipid peroxidation. We also found an increase in total sperm defects, acrosome and membrane damages in the group with the highest susceptibility to induced lipid peroxidation. Additionally, the low mitochondrial membrane potential, susceptible to chromatin fragmentation and the PRM1 mRNA were increased in the group showing higher susceptibility to lipid peroxidation. Ram sperm susceptibility to lipid peroxidation may compromise sperm quality and interfere with the oxidative homeostasis by oxidative stress, which may be the main cause of chromatin damage in ram sperm. (AU)

• Processo FAPESP: 13/05025-1 - Mensuração das enzimas antioxidantes no sêmen de ovinos: concentrações plasmáticas e celulares
• Beneficiário: Thais Rose dos Santos Hamilton
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/11231-8 - Estudo dos fatores envolvidos na fragmentação de DNA dos espermatozóides em ovinos.
• Beneficiário: Thais Rose dos Santos Hamilton
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/58487-1 - Influencia dos indices espermaticos de protamina sobre a fertilidade in vitro em bovinos.
• Beneficiário: Mayra Elena Ortiz D' Avila Assumpcao
• Modalidade de apoio: Auxílio à Pesquisa - Regular