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The HEX 110 Hexamerin Is a Cytoplasmic and Nucleolar Protein in the Ovaries of Apis mellifera

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Autor(es):
Martins, Juliana Ramos [1] ; Gentile Bitondi, Marcia Maria [2]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 11, n. 3 MAR 8 2016.
Citações Web of Science: 4
Resumo

Hexamerins are insect storage proteins abundantly secreted by the larval fat body into the haemolymph. The canonical role of hexamerins consists of serving as an amino acid reserve for development toward the adult stage. However, in Apis mellifera, immunofluorescence assays coupled to confocal laser-scanning microscopy, and high-throughput sequencing, have recently shown the presence of hexamerins in other organs than the fat body. These findings have led us to study these proteins with the expectation of uncovering additional functions in insect development. We show here that a honeybee hexamerin, HEX 110, localizes in the cytoplasm and nucleus of ovarian cells. In the nucleus of somatic and germline cells, HEX 110 colocalized with a nucleolar protein, fibrillarin, suggesting a structural or even regulatory function in the nucleolus. RNase A provoked the loss of HEX 110 signals in the ovarioles, indicating that the subcellular localization depends on RNA. This was reinforced by incubating ovaries with pyronin Y, a RNA-specific dye. Together, the colocalization with fibrillarin and pyronin Y, and the sensitivity to RNase, highlight unprecedented roles for HEX110 in the nucleolus, the nuclear structure harbouring the gene cluster involved in ribosomal RNA production. However, the similar patterns of HEX 110 foci distribution in the active and inactive ovaries of queens and workers preclude its association with the functional status of these organs. (AU)

Processo FAPESP: 11/03171-5 - Análise causal do desenvolvimento de Apis mellifera - genes reguladores e redes hierárquicas de expressão gênica na especificação de tecidos e órgãos
Beneficiário:Zilá Luz Paulino Simões
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 12/16040-9 - Hexamerinas de Apis mellifera: interação entre as subunidades, com o hormônio juvenil e com estruturas e proteínas nucleares
Beneficiário:Juliana Ramos Martins
Linha de fomento: Bolsas no Brasil - Pós-Doutorado