Insights into the plant polysaccharide degradation potential of the xylanolytic yeast Pseudozyma brasiliensis

Autor(es)

Kaupert Neto, Antonio Adalberto ^[1] ; Borin, Gustavo Pagotto ^[1] ; Goldman, Gustavo Henrique ^{[2, 1]} ; de Lima Damasio, Andre Ricardo ^{[1, 3]} ; de Castro Oliveira, Juliana Velasco ^[1]

Afiliação do(s) autor(es)

^[1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, Av Giuseppe Maximo Scolfaro 10000, Caixa Postal 61, BR-13083970 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Estadual Campinas, Dept Bioquim & Biol Tecidual, Inst Biol, Rua Monteiro Lobato 255, Caixa Postal 6109, BR-13083970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEMS Yeast Research; v. 16, n. 2 MAR 2016.
• Citações Web of Science: 1

Resumo

In second-generation (2G) bioethanol production, plant cell-wall polysaccharides are broken down to release fermentable sugars. The enzymes of this process are classified as carbohydrate-active enzymes (CAZymes) and contribute substantially to the cost of biofuel production. A novel basidiomycete yeast species, Pseudozyma brasiliensis, was recently discovered. It produces an endo-beta-1,4-xylanase with a higher specific activity than other xylanases. This enzyme is essential for the hydrolysis of biomass-derived xylan and has an important role in 2G bioethanol production. In spite of the P. brasiliensis biotechnological potential, there is no information about how it breaks down polysaccharides. For the first time, we characterized the secretome of P. brasiliensis grown on different carbon sources (xylose, xylan, cellobiose and glucose) and also under starvation conditions. The growth and consumption of each carbohydrate and the activity of the CAZymes of culture supernatants were analyzed. The CAZymes found in its secretomes, validated by enzymatic assays, have the potential to hydrolyze xylan, mannan, cellobiose and other polysaccharides. The data show that this yeast is a potential source of hydrolases, which can be used for biomass saccharification. (AU)

• Processo FAPESP: 13/18910-3 - Secreção de glicoproteínas heterólogas em Aspergillus: efeito do padrão de glicosilação em parâmetros funcionais de glicosil hidrolases
• Beneficiário: André Ricardo de Lima Damasio
• Modalidade de apoio: Bolsas no Brasil - Programa BIOEN - Jovens Pesquisadores
• Processo FAPESP: 14/11766-7 - Caracterização de novos fatores de transcrição de Trichoderma reesei envolvidos na degradação da biomassa lignocelulósica
• Beneficiário: Juliana Velasco de Castro Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 15/08222-8 - Análise da co-regulação transcricional e identificação de genes de interesse biotecnológico em Trichoderma reesei
• Beneficiário: Gustavo Pagotto Borin
• Modalidade de apoio: Bolsas no Brasil - Doutorado