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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genomic survey and expression analysis of DNA repair genes in the genus Leptospira

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Autor(es):
Martins-Pinheiro, Marinalva [1, 2] ; Schons-Fonseca, Luciane [3] ; da Silva, Josefa B. [3] ; Domingos, Renan H. [3] ; Santos Momo, Leonardo Hiroyuki ; Quirino Simoes, Ana Carolina [4] ; Ho, Paulo Lee [3, 5] ; da Costa, Renata M. A. [6]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Escola Politecn, Dept Engn Quim, Sao Paulo, SP - Brazil
[3] Inst Butantan, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil
[4] Univ Fed ABC, Ctr Engn Modelagem & Ciencias Sociais Aplicadas, BR-09210170 Santo Andre - Brazil
[5] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil
[6] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Molecular Genetics and Genomics; v. 291, n. 2, p. 703-722, APR 2016.
Citações Web of Science: 5
Resumo

Leptospirosis is an emerging zoonosis with important economic and public health consequences and is caused by pathogenic leptospires. The genus Leptospira belongs to the order Spirochaetales and comprises saprophytic (L. biflexa), pathogenic (L. interrogans) and host-dependent (L. borgpetersenii) members. Here, we present an in silico search for DNA repair pathways in Leptospira spp. The relevance of such DNA repair pathways was assessed through the identification of mRNA levels of some genes during infection in animal model and after exposition to spleen cells. The search was performed by comparison of available Leptospira spp. genomes in public databases with known DNA repair-related genes. Leptospires exhibit some distinct and unexpected characteristics, for instance the existence of a redundant mechanism for repairing a chemically diverse spectrum of alkylated nucleobases, a new mutS-like gene and a new shorter version of uvrD. Leptospira spp. shares some characteristics from Gram-positive, as the presence of PcrA, two RecQ paralogs and two SSB proteins; the latter is considered a feature shared by naturally competent bacteria. We did not find a significant reduction in the number of DNA repair-related genes in both pathogenic and host-dependent species. Pathogenic leptospires were enriched for genes dedicated to base excision repair and non-homologous end joining. Their evolutionary history reveals a remarkable importance of lateral gene transfer events for the evolution of the genus. Up-regulation of specific DNA repair genes, including components of SOS regulon, during infection in animal model validates the critical role of DNA repair mechanisms for the complex interplay between host/pathogen. (AU)

Processo FAPESP: 10/51365-0 - Contribuição ao conhecimento de Leptospira interrogans serovar Copenhageni utilizando a informação genômica
Beneficiário:Paulo Lee Ho
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 13/16451-1 - Avaliação in vitro da contribuição de CCL2/MCP-1 e a importância de quimiocinas nos mecanismos de resistência e susceptibilidade à infecção por Leptospira sp.
Beneficiário:Josefa Bezerra da Silva
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/17123-2 - Identificação e caracterização molecular de genes envolvidos na resposta do agente infeccioso Leptospira interrogans ao estresse oxidativo
Beneficiário:Renata Maria Augusto da Costa
Linha de fomento: Auxílio à Pesquisa - Regular